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LHPP Polyclonal antibody
LHPP Polyclonal Antibody for IF, IHC, WB,ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human, mouse, rat
Applications
WB, IHC, IF, ELISA
Conjugate
Unconjugated
Cat no : 15759-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | mouse liver tissue, human liver tissue, human brain tissue, A549 cells, HeLa cells, rat liver tissue, A431 cells, HepG2 cells |
Positive IHC detected in | human liver cancer tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Positive IF detected in | Hela cells |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:500-1:3000 |
Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
Immunofluorescence (IF) | IF : 1:10-1:100 |
Sample-dependent, check data in validation data gallery |
Published Applications
KD/KO | See 4 publications below |
WB | See 8 publications below |
IHC | See 5 publications below |
IF | See 1 publications below |
Product Information
15759-1-AP targets LHPP in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Cited Reactivity | human |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LHPP fusion protein Ag8375 |
Full Name | phospholysine phosphohistidine inorganic pyrophosphate phosphatase |
Calculated molecular weight | 23 kDa, 29 kDa |
Observed molecular weight | 23-32 kDa |
GenBank accession number | BC007324 |
Gene symbol | LHPP |
Gene ID (NCBI) | 64077 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for LHPP antibody 15759-1-AP | Download protocol |
IHC protocol for LHPP antibody 15759-1-AP | Download protocol |
IF protocol for LHPP antibody 15759-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Mol Cancer YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer. | ||
Oncol Rep Tumor suppressor LHPP regulates the proliferation of colorectal cancer cells via the PI3K/AKT pathway. | ||
J Physiol Biochem Tumor suppressor LHPP suppresses cell proliferation and epithelial-mesenchymal transition in hepatocellular carcinoma cell lines.
| ||
Biosci Rep LHPP suppresses the bladder cancer cell proliferation and growth via inactivating AKT/p65 signaling pathway.
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