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  • KD/KO Validated

LHPP Polyclonal antibody

LHPP Polyclonal Antibody for IF, IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 15759-1-AP

Synonyms

hLHPP, LHPP



Tested Applications

Positive WB detected inmouse liver tissue, human liver tissue, human brain tissue, A549 cells, HeLa cells, rat liver tissue, A431 cells, HepG2 cells
Positive IHC detected inhuman liver cancer tissue, human kidney tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inHela cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Product Information

15759-1-AP targets LHPP in WB, IHC, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LHPP fusion protein Ag8375
Full Name phospholysine phosphohistidine inorganic pyrophosphate phosphatase
Calculated molecular weight 23 kDa, 29 kDa
Observed molecular weight 23-32 kDa
GenBank accession numberBC007324
Gene symbol LHPP
Gene ID (NCBI) 64077
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for LHPP antibody 15759-1-APDownload protocol
IHC protocol for LHPP antibody 15759-1-APDownload protocol
IF protocol for LHPP antibody 15759-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Cell Cycle

LHPP inhibits hepatocellular carcinoma cell growth and metastasis.

Authors - Lijuan Liao
humanWB

Biomed Res Int

The Significance of Circular RNA DDX17 in Prostate Cancer.

Authors - Qi Lin
humanWB

Aging (Albany NY)

The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis.

Authors - Xuhui Yang
  • KD Validated
humanWB,IHC

Biosci Rep

LHPP suppresses the bladder cancer cell proliferation and growth via inactivating AKT/p65 signaling pathway.

Authors - Yansheng Li
  • KD Validated
humanWB,IHC,IF

Oncol Rep

Tumor suppressor LHPP regulates the proliferation of colorectal cancer cells via the PI3K/AKT pathway.

Authors - Bin Hou
humanIHC

Mol Cancer

YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.

Authors - Jiangfeng Li