LHPP Polyclonal antibody

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• KD/KO Validated

LHPP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 15759-1-AP Copied!

Publications (18)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, hLHPP, EC:3.1.3.-

• 

  WB analysis using 15759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 15759-1-AP

  WB result of LHPP antibody (15759-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LHPP transfected A549 cells.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of mouse liver using 15759-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis using 15759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human liver using 15759-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of human brain using 15759-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A549 using 15759-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human liver cancer using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 10x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human kidney using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 40x lens).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of HeLa using 15759-1-AP

  Immunofluorescent analysis of Hela cells, using LHPP antibody 15759-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, human brain tissue, A549 cells, HeLa cells, rat liver tissue, A431 cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15759-1-AP targets LHPP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag8375

  Product name: Recombinant human LHPP protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-210 aa of BC007324

  Sequence: MAPWGKRLAGVRGVLLDISGVLYDSGAGGGTAIAGSVEAVARLKRSRLKVRFCTNESQKSRAELVGQLQRLGFDISEQEVTAPAPAACQILKERGLRPYLLIHDGVRSEFDQIDTSNPNCVVIADAGESFSYQNMNNAFQVLMELEKPVLISLGKGRYYKETSGLMLDVGPYMKALEYACGIKAEVVGKPSPEFFKSALQAIGVEAHQAQ

• Full Name: phospholysine phosphohistidine inorganic pyrophosphate phosphatase
• Calculated Molecular Weight: 23 kDa, 29 kDa
• Observed Molecular Weight: 23-32 kDa
• GenBank Accession Number: BC007324
• Gene Symbol: LHPP
• Gene ID (NCBI): 64077
• RRID: AB_2296904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H008
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

The LHPP antibody (Cat# 15759-1-AP) has 18 total citations published in journals including Molecular Cancer, Cell Death & Disease, Cancer Letters, Cell Reports, FASEB Journal, Aging, International Immunopharmacology, and BMC Medical Genomics. Research fields cover oncology (prostate, gastric, hepatocellular, nasopharyngeal, colorectal, bladder, and lung cancers), ferroptosis, liver fibrosis, neurogenesis, and colitis, involving AKT, PI3K/AKT/mTOR, EGFR, and TGF-β/Smad3 signaling pathways.

Automatically generated from the 18 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	IHC	Mol Cancer; YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.; Authors - Jiangfeng Li
human	WB,IHC	Cell Death Dis; Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway; Authors - Guoqing Xie
human	IHC	Cell Death Dis; Targeting LHPP in neoadjuvant chemotherapy resistance of gastric cancer: insights from single-cell and multi-omics data on tumor immune microenvironment and stemness characteristics; Authors - You-Xin Gao
human	IHC	Cancer Lett; HDAC4 mediated LHPP deacetylation enhances its destabilization and promotes the proliferation and metastasis of nasopharyngeal carcinoma; Authors - Xueshuo Sun
mouse	WB,IF	Cell Rep; LHPP-mediated inorganic pyrophosphate hydrolysis-driven lysosomal acidification in astrocytes regulates adult neurogenesis; Authors - Longze Sha ; KO Validated
human	WB	Aging (Albany NY); The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis.; Authors - Xuhui Yang ; KD Validated