LHPP Polyclonal antibody

• Featured Product
• KD/KO Validated

LHPP Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Conjugate

Unconjugated

Publications

16

Cat no : 15759-1-AP

Synonyms

Phospholysine phosphohistidine inorganic pyrophosphate phosphatase, hLHPP, EC:3.1.3.-

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Validation Data Gallery

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  WB analysis using 15759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 15759-1-AP

  WB result of LHPP antibody (15759-1-AP; 1:3000; incubated at room temperature for 1.5 hours) with sh-Control and sh-LHPP transfected A549 cells.

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  WB analysis of mouse liver using 15759-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 15759-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human liver using 15759-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 15759-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 15759-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 15759-1-AP (LHPP antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15759-1-AP (LHPP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 15759-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15759-1-AP (LHPP antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 15759-1-AP

  Immunofluorescent analysis of Hela cells, using LHPP antibody 15759-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: mouse liver tissue, human liver tissue, human brain tissue, A549 cells, HeLa cells, rat liver tissue, A431 cells, HepG2 cells
• Positive IHC detected in: human liver cancer tissue, human kidney tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: Hela cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 5 publications below
• WB: See 13 publications below
• IHC: See 8 publications below
• IF: See 2 publications below

Product Information

15759-1-AP targets LHPP in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LHPP fusion protein Ag8375
• Full Name: phospholysine phosphohistidine inorganic pyrophosphate phosphatase
• Calculated Molecular Weight: 23 kDa, 29 kDa
• Observed Molecular Weight: 23-32 kDa
• GenBank Accession Number: BC007324
• Gene Symbol: LHPP
• Gene ID (NCBI): 64077
• RRID: AB_2296904
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	IHC	Mol Cancer; YTHDF2 mediates the mRNA degradation of the tumor suppressors to induce AKT phosphorylation in N6-methyladenosine-dependent way in prostate cancer.; Authors - Jiangfeng Li
human	WB,IHC	Cell Death Dis; Phosphatase LHPP confers prostate cancer ferroptosis activation by modulating the AKT-SKP2-ACSL4 pathway; Authors - Guoqing Xie
human	WB	Biochim Biophys Acta Mol Basis Dis; Correlated with better prognosis, CSTA inhibits metastasis of nasopharyngeal carcinoma cells via suppressing AKT signaling through promoting METTL3 degradation; Authors - Zhi-Jie Liu
human	WB	Aging (Albany NY); The long intergenic noncoding RNA GAS5 reduces cisplatin-resistance in non-small cell lung cancer through the miR-217/LHPP axis.; Authors - Xuhui Yang ; KD Validated
human	WB	Cell Cycle; LHPP inhibits hepatocellular carcinoma cell growth and metastasis.; Authors - Lijuan Liao
human	WB,IHC,IF	Oncol Rep; Tumor suppressor LHPP regulates the proliferation of colorectal cancer cells via the PI3K/AKT pathway.; Authors - Bin Hou