LIFR Recombinant antibody

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  WB analysis using 85827-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85827-2-RR (LIFR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

LIFR Uni-rAb^TM Recombinant Antibody for WB, ELISA

Cat No. 85827-2-RR

Clone No.250110B11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

CD118, LIF R, LIF receptor, LIF-R, SJS2

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Tested Applications

• Positive WB detected in: HeLa cells, PC-3 cells, PANC-1 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

85827-2-RR targets LIFR in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LIFR fusion protein Eg2599
• Full Name: leukemia inhibitory factor receptor alpha
• Calculated Molecular Weight: 124 kDa
• Observed Molecular Weight: 190 kDa, 170 kDa
• GenBank Accession Number: NM_001127671.1
• Gene Symbol: LIFR
• Gene ID (NCBI): 3977
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P42702-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LIFR, also known as CD118, is a subunit of a receptor for leukemia inhibitory factor (LIF). LIF is a pleiotropic cytokine of the interleukin-6 family that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. LIFR is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of LIF. The high-affinity complex also binds a related cytokine, oncostatin M (PMID: 8999038). LIFR has also been identified as a breast cancer metastasis suppressor that functions through the HIPPO-YAP pathway (PMID: 23001183). LIFR appeared as doublets with 190 and 170 kDa (PMID: 10858440). They represented different glycosylated forms of the LIFR in different steps of protein maturation.