KD/KO Validated

LIG1 Monoclonal antibody

LIG1 Monoclonal Antibody for WB, ELISA

Cat No. 67840-1-Ig

Clone No. 3D9G8

Publications (1)

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Host / Isotype

Mouse / IgG2b

Reactivity

human, mouse, rat

Applications

WB, CoIP, ELISA

Synonyms

DNA ligase I, 3D9G8, DNA ligase 1, EC:6.5.1.1, ligase I, DNA, ATP dependent

Formulation: PBS, Azide, Glycerol

PBS, Azide, Glycerol

PBS Only

Conjugate: Unconjugated

Unconjugated

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WB analysis using 67840-1-Ig
Various lysates were subjected to SDS PAGE followed by western blot with 67840-1-Ig (LIG1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Lamin B1 Monoclonal antibody (HRP-66095) as loading control.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
WB analysis of HepG2 using 67840-1-Ig
WB result of LIG1 antibody (67840-1-Ig; 1:1200; incubated at room temperature for 1.5 hours) with sh-Control and sh-LIG1 transfected HepG2 cells.
This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Overview

Tested Applications
Product Information
Publications (1)
Protocols

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MSDS SDS

Tested Applications

Positive WB detected in

HepG2 cells, HeLa cells, Jurkat cells, MOLT-4 cells, K-562 cells, HSC-T6 cells

Recommended dilution

Application	Dilution
Western Blot (WB)	WB : 1:5000-1:50000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

67840-1-Ig targets LIG1 in WB, CoIP, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity	human, mouse, rat
Cited Reactivity	human
Host / Isotype	Mouse / IgG2b
Class	Monoclonal
Type	Antibody
Immunogen	CatNo: Ag12489 Product name: Recombinant human LIG1 protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 670-919 aa of BC108318 Sequence: LVREPLSRRRQLLRENFVETEGEFVFATSLDTKDIEQIAEFLEQSVKDSCEGLMVKTLDVDATYEIAKRSHNWLKLKKDYLDGVGDTLDLVVIGAYLGRGKRAGRYGGFLLASYDEDSEELQAICKLGTGFSDEELEEHHQSLKALVLPSPRPYVRIDGAVIPDHWLDPSAVWEVKCADLSLSPIYPAARGLVDSDKGISLRFPRFIRVREDKQPEQATTSAQVACLYRKQSQIQNQQGEDSGSDPEDTY Predict reactive species
Full Name	ligase I, DNA, ATP-dependent
Calculated Molecular Weight	919 aa, 102 kDa
Observed Molecular Weight	130 kDa
GenBank Accession Number	BC108318
Gene Symbol	LIG1
Gene ID (NCBI)	3978
RRID	AB_2918602
Conjugate	Unconjugated
Form	Liquid
Purification Method	Protein A purification
UNIPROT ID	P18858
Storage Buffer	PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
Storage Conditions	Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

DNA ligase I (LIG1) joins DNA strand breaks during DNA replication and repair transactions and contributes to genome integrity. 67840-1-Ig is raised agains the C-terminal 670-919 aa residues of the human DNA ligase 1.

Protocols

Product Specific Protocols
WB protocol for LIG1 antibody 67840-1-Ig	Download protocol

Standard Protocols
Click here to view our Standard Protocols

Publications

What published studies show

The LIG1 Monoclonal antibody (3D9G8), catalog number 67840-1-Ig, has a total of 1 citation. The cited publication appeared in bioRxiv (2023), titled "Distinct functions of EHMT1 and EHMT2 in cancer chemotherapy and immunotherapy." The research field associated with this citation is cancer biology, specifically focusing on chemotherapy and immunotherapy mechanisms. The antibody was used in WB and CoIP applications on human samples.