Anti-Human LILRA5/CD85f Rabbit Recombinant Antibody

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  FC experiment of human PBMCs using 98477-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRA5/CD85f Rabbit RecAb (98477-1-RR, Clone:250298G11) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were then stained with APC Anti-Human CD14 Rabbit Recombinant Antibody (APC-98040, Clone: 230332D7). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

• 

  FC experiment of human PBMCs using 98477-1-RR

  1x10^6 human PBMCs were surface stained with 0.25 ug Anti-Human LILRA5/CD85f Rabbit RecAb (98477-1-RR, Clone: 250298G11) (red) or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue), and PE-Conjugated Goat Anti-Rabbit IgG(H+L). Cells were incubated with FC Receptor Block prior to fixing and staining. Cells were not fixed.

LILRA5/CD85f Uni-rAb^TM Recombinant Antibody for FC

Cat No. 98477-1-RR

Clone No.250298G11

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

FC

Synonyms

LILRA5, CD85, CD85 antigen-like family member F, CD85f, ILT 11

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Tested Applications

• Positive FC detected in: human PBMCs

Recommended dilution

• Flow Cytometry (FC): FC : 0.25 ug per 10^6 cells in a 100 µl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

98477-1-RR targets LILRA5/CD85f in FC applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: LILRA5/CD85f fusion protein Eg2737
• Full Name: leukocyte immunoglobulin-like receptor, subfamily A (with TM domain), member 5
• Calculated Molecular Weight: 33 kDa
• GenBank Accession Number: NM_021250.4
• Gene Symbol: LILRA5
• Gene ID (NCBI): 353514
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: A6NI73-1
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2 - 8°C. Stable for one year after shipment.

Background Information

LILRA5, also named as CD85f, ILT11, LILRB7 or LIR9, is a member of the leukocyte immunoglobulin-like receptor (LIR) family, which is a group of immune inhibitory and stimulatory receptors (PMID: 15304001). There are two membrane-bound and two soluble forms of LILRA5. The transmembrane LILRA5 contains a short cytoplasmic domain and a charged arginine residue within the transmembrane region (PMID: 19009525). It is mostly expressed in myeloid cells, including monocytes and neutrophils (PMID: 12393390). LILRA5 may play a role in triggering innate immune responses. It does not function in class I MHC antigens recognition (PMID: 16675463).