LIN41 Polyclonal antibody

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  WB analysis of mouse kidney using 55003-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 55003-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of DU 145 using 55003-1-AP

  DU 145 cells were subjected to SDS PAGE followed by western blot with 55003-1-AP (LIN41 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 55003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55003-1-AP (LIN41 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 55003-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 55003-1-AP (LIN41 antibody) at dilution of 1:50 (under 40x lens).

LIN41 Polyclonal Antibody for WB, IHC, ELISA

Cat No. 55003-1-AP

Publications(3)

Reviews (1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Synonyms

TRIM71, RING-type E3 ubiquitin transferase TRIM71, Protein lin-41 homolog, Protein lin 41 homolog, LIN 41

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Tested Applications

• Positive WB detected in: mouse kidney tissue, DU 145 cells, NIH/3T3 cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 1 publications below

Product Information

55003-1-AP targets LIN41 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: tripartite motif-containing 71
• Calculated Molecular Weight: 93 kDa
• Observed Molecular Weight: 93 kDa
• GenBank Accession Number: NM_001039111
• Gene Symbol: LIN41
• Gene ID (NCBI): 131405
• RRID: AB_2616313
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q2Q1W2
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

TRIM71, also named as LIN41, belongs to the TRIM/RBCC family. It may be involved in controlling the timing of organ formation during development. The antibody is specific to TRIM71.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB	Cell Cycle; TRIM71 binds to IMP1 and is capable of positive and negative regulation of target RNAs.; Authors - Daniel J Foster
human	WB	Cell Mol Gastroenterol Hepatol; HDLBP promotes hepatocellular carcinoma proliferation and sorafenib resistance by suppressing Trim71-dependent RAF1 degradation; Authors - Jingsheng Yuan
human,mouse	IHC	Mol Carcinog; Circ_0007429/miR-637/TRIM71/Ago2 axis participates in the regulation of proliferation, migration, invasion, apoptosis, and aerobic glycolysis of HCC; Authors - Lilan Fan

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Daniel (Verified Customer) (10-26-2022)

This antibody produces very high background, using either milk or Licor blocking buffers, PVDF or nitrocellulose.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HeLa, MT4 cells

LIN41 Antibody Western Blot validation (1:500 dilution) in HeLa, MT4 cells (Cat no:55003-1-AP)