LIPG Monoclonal antibody

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  WB analysis using 67434-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

• 

  WB analysis of human placenta using 67434-1-Ig

  human placenta tissue were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 67434-1-Ig

  HepG2 cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HaCaT using 67434-1-Ig

  HaCaT cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-29 using 67434-1-Ig

  HT-29 cells were subjected to SDS PAGE followed by western blot with 67434-1-Ig (LIPG antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 67434-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67434-1-Ig (LIPG antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 67434-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 67434-1-Ig (LIPG antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

LIPG Monoclonal Antibody for WB, IHC, ELISA

Cat No. 67434-1-Ig

Clone No.1B10B10

Publications(1)

Host / Isotype

Mouse / IgG1

Reactivity

Human and More (1)

Applications

WB, IHC, CoIP, ELISA

Synonyms

EDL, EL, Endothelial lipase, lipase, endothelial, LIPG, PRO719

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Tested Applications

• Positive WB detected in: Caco-2 cells, HaCaT cells, HepG2 cells, HT-29 cells, human placenta tissue, MCF-7 cells, MDA-MB-468 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 1 publications below
• CoIP: See 1 publications below

Product Information

67434-1-Ig targets LIPG in WB, IHC, CoIP, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag29482

  Product name: Recombinant human LIPG protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 150-500 aa of BC060825

  Sequence: DWLQEKDDFSLGNVHLIGYSLGAHVAGYAGNFVKGTVGRITGLDPAGPMFEGADIHKRLSPDDADFVDVLHTYTRSFGLSIGIQMPVGHIDIYPNGGDFQPGCGLNDVLGSIAYGTITEVVKCEHERAVHLFVDSLVNQDKPSFAFQCTDSNRFKKGICLSCRKNRCNSIGYNAKKMRNKRNSKMYLKTRAGMPFRVYHYQMKIHVFSYKNMGEIEPTFYVTLYGTNADSQTLPLEIVERIEQNATNTFLVYTEEDLGDLLKIQLTWEGASQSWYNLWKEFRSYLSQPRNPGRELNIRRIRVKSGETQRKLTFCTEDPENTSISPGQELWFRKCRDGWRMKNETSPTVELP

• Full Name: lipase, endothelial
• Calculated Molecular Weight: 57 kDa
• Observed Molecular Weight: 57 and 42 kDa
• GenBank Accession Number: BC060825
• Gene Symbol: LIPG
• Gene ID (NCBI): 9388
• RRID: AB_2882672
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: Q9Y5X9
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
human	WB,IHC,CoIP	Cancer Gene Ther; ZDHHC1 downregulates LIPG and inhibits colorectal cancer growth via IGF2BP1 Palmitoylation; Authors - Qun Zhang ; KD Validated