Tested Applications
| Positive WB detected in | mouse brain tissue, rat brain tissue |
| Positive IHC detected in | mouse brain tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20678-1-AP targets LIS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
Peptide Predict reactive species |
| Full Name | platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa |
| Calculated Molecular Weight | 47 kDa |
| Observed Molecular Weight | 45-50 kDa |
| GenBank Accession Number | NM_000430 |
| Gene Symbol | LIS1 |
| Gene ID (NCBI) | 5048 |
| RRID | AB_2878723 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P43034 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria. The LIS1 protein has a calculated molecular mass of ~45 kDa.(PMID: 29150182, PMID: 29562183, PMID: 15800059)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LIS1 antibody 20678-1-AP | Download protocol |
| WB protocol for LIS1 antibody 20678-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LIS1 Polyclonal antibody (Cat# 20678-1-AP) has 4 total citations. These appear in Nature Communications, Communications Biology, iScience, and Iranian Journal of Biotechnology. The associated research fields span neuroscience (Zika virus–induced neurological damage, Parkinson's disease microglial inflammation, and microtubule dynamics in axon terminals) and oncology (cholangiocarcinoma cell proliferation and invasion). Cited applications include Western blot and immunohistochemistry in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Nat Commun DISC1 Protects Against Zika Virus Infection and Long-Term Neurological Damage Through AMPK-mTOR-Mediated Autophagy | ||
Commun Biol Targeting microglial inflammation in Parkinson's disease: irisin activates PAFAH1B1-RAGE ubiquitination and TFEB-dependent autophagy to alleviate neurodegeneration. | ||
iScience NudC regulated Lis1 stability is essential for the maintenance of dynamic microtubule ends in axon terminals | ||
Iran J Biotechnol MiR-582 Down-Regulates Lissencephaly-1 (LIS1) via P-Akt and MMP-2 to Inhibit Cholangiocarcinoma Cell Proliferation and Invasion |
Reviews
What customers say
The single review for this antibody reports a 4-star rating from a Western Blot application on mouse cortex tissue. The user found it to be a good antibody with quite low background, noting that a 1/2000 dilution could be sufficient, though they used 1/1000. Overall, the feedback is positive, highlighting strong performance and low background in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Aurélie (Verified Customer) (06-13-2019) | Good antibody in WB with quite low background, 1/2000 could be enough
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