LIS1 Polyclonal antibody

LIS1 Polyclonal Antibody for WB, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 20678-1-AP

Synonyms

LIS 1, LIS1, LIS2, Lissencephaly 1 protein, MDCR, MDS, PAF AH 45 kDa subunit, PAF AH alpha, PAFAH, PAFAH alpha, PAFAH1B1, PAFAHA

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Validation Data Gallery

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  WB analysis using 20678-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20678-1-AP (LIS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20678-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20678-1-AP (LIS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 20678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20678-1-AP (LIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 20678-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20678-1-AP (LIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IHC detected in: mouse brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below
• IHC: See 1 publications below

Product Information

20678-1-AP targets LIS1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_000430
• Gene Symbol: LIS1
• Gene ID (NCBI): 5048
• RRID: AB_2878723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria. The LIS1 protein has a calculated molecular mass of ~45 kDa.(PMID: 29150182, PMID: 29562183, PMID: 15800059)

Publications

Species	Application	Title
human,mouse	WB,IHC	Iran J Biotechnol; MiR-582 Down-Regulates Lissencephaly-1 (LIS1) via P-Akt and MMP-2 to Inhibit Cholangiocarcinoma Cell Proliferation and Invasion; Authors - Guochao Liu

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aurélie (Verified Customer) (06-13-2019)

Good antibody in WB with quite low background, 1/2000 could be enough

• Applications: Western Blot,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: Mouse Cortex

LIS1 Antibody Western Blot, validation (1/1000 dilution) in Mouse Cortex (Cat no:20678-1-AP)