LIS1 Polyclonal antibody, PBS Only

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• LIS1 Polyclonal antibody, PBS Only (20678-1-PBS)

• 

  WB analysis using 20678-1-AP (same clone as 20678-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20678-1-AP (LIS1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20678-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 20678-1-AP (same clone as 20678-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20678-1-AP (LIS1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20678-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20678-1-AP (same clone as 20678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20678-1-AP (LIS1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20678-1-PBS in a different storage buffer formulation.

• 

  IHC staining of mouse brain using 20678-1-AP (same clone as 20678-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20678-1-AP (LIS1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20678-1-PBS in a different storage buffer formulation.

LIS1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 20678-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

20678-1-PBS targets LIS1 in WB, IHC, Indirect ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: platelet-activating factor acetylhydrolase, isoform Ib, alpha subunit 45kDa
• Calculated Molecular Weight: 47 kDa
• Observed Molecular Weight: 45-50 kDa
• GenBank Accession Number: NM_000430
• Gene Symbol: LIS1
• Gene ID (NCBI): 5048
• RRID: AB_2878723
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43034
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Platelet-activating factor acetylhydrolase 1B subunit alpha (LIS1), a critical mediator of neuronal migration in developing brain, is expressed throughout life. LIS1 forms a homodimer that interacts directly with the dynein complex and is essential for its enrichment at dynamic microtubule plus ends in mammalian cells. In addition to regulating the association of dynein with microtubule plus ends, LIS1 is required for efficient transport of many dynein-associated cargos and proper localization of Golgi complexes, endosomes, lysosomes, and mitochondria. The LIS1 protein has a calculated molecular mass of ~45 kDa.(PMID: 29150182, PMID: 29562183, PMID: 15800059)