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LNPEP Polyclonal antibody, PBS Only (55154-1-PBS)

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WB analysis of human brain using 55154-1-AP (same clone as 55154-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
WB analysis of human heart using 55154-1-AP (same clone as 55154-1-PBS)
human heart tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
WB analysis of COLO 320 using 55154-1-AP (same clone as 55154-1-PBS)
COLO 320 cells were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
WB analysis of human brain using 55154-1-AP (same clone as 55154-1-PBS)
human brain tissue were subjected to SDS PAGE followed by western blot with 55154-1-AP (LNPEP antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 55154-1-AP (same clone as 55154-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
IHC staining of human heart using 55154-1-AP (same clone as 55154-1-PBS)
Immunohistochemical analysis of paraffin-embedded human heart using 55154-1-AP (LNPEP antibody) at dilution of 1:50 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.
IHC staining of human kidney using 55154-1-AP (same clone as 55154-1-PBS)
Immunohistochemical analysis of paraffin-embedded human kidney using 55154-1-AP (LNPEP antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 55154-1-PBS in a different storage buffer formulation.

LNPEP Polyclonal antibody, PBS Only

Name: LNPEP Polyclonal antibody, PBS Only
Brand: Proteintech
SKU: 55154-1-PBS

LNPEP Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 55154-1-PBS

Review Product

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

Formulation: PBS Only

PBS, Azide, Glycerol

Conjugate: Unconjugated

Size/Concentration:

-/ -

Freight/Packing: -

Quantity

- +

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Overview

Tested Applications
Product Information

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MSDS SDS

Product Information

55154-1-PBS targets LNPEP in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

Tested Reactivity	human
Host / Isotype	Rabbit / IgG
Class	Polyclonal
Type	Antibody
Immunogen	Peptide Predict reactive species
Full Name	leucyl/cystinyl aminopeptidase
Calculated Molecular Weight	117 kDa
Observed Molecular Weight	117-125 kDa
GenBank Accession Number	NM_005575
Gene Symbol	LNPEP
Gene ID (NCBI)	4012
RRID	AB_10860904
Conjugate	Unconjugated
Form	Liquid
Purification Method	Antigen affinity purification
UNIPROT ID	Q9UIQ6
Storage Buffer	PBS only, pH 7.3.
Storage Conditions	Store at -80°C.

Background Information

LNPEP, also named as OTASE, IRAP, P-LAP and Otase, may be involved in the inactivation of neuronal peptides in the brain. LNPEP cleaves Met-enkephalin and dynorphin. It binds angiotensin IV and may be the angiotensin IV receptor in the brain. It can be detected a protein with molecular mass of 165 kDa in several human tissues including heart, small intestine, kidney and 140 kDa in brain. LNPEP contains 18 potential N-glycosylation sites so that the differential usage of the glycosylation site in the brain causes the difference in the molecular mass of the enzyme.(PMID:11389728). Two forms of LNPEP are purified from human placenta: one is a monomeric, 61-kDa protein and the other is a heterodimeric protein with 64- and 97-kDa subunits(PMID:10601849). It can form a homodimer of 280 kDa(PMID:10601849). This antibody is specific to LNPEP.