Tested Applications
| Positive WB detected in | HeLa cells, Jurkat cells, K-562 cells |
| Positive IHC detected in | human lung cancer tissue, human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17169-1-AP targets LOH12CR1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10695 Product name: Recombinant human LOH12CR1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-196 aa of BC013668 Sequence: MGSEQSSEAESRPNDLNSSVTPSPAKHRAKMDDIVVVAQGSQASRNVSNDPDVIKLQEIPTFQPLLKGLLSGQTSPTNAKLEKLDSQQVLQLCLRYQDHLHQCAEAVAFDQNALVKRIKEMDLSVETLFSFMQERQKRYAKYAEQIQKVNEMSAILRRIQMGIDQTVPLLDRLNSMLPEGERLEPFSMKPDRELRL Predict reactive species |
| Full Name | loss of heterozygosity, 12, chromosomal region 1 |
| Calculated Molecular Weight | 196 aa, 22 kDa |
| Observed Molecular Weight | 17-22 kDa |
| GenBank Accession Number | BC013668 |
| Gene Symbol | LOH12CR1 |
| Gene ID (NCBI) | 118426 |
| RRID | AB_2137150 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q969J3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LOH12CR1, also named as BORCS5, is part of the BORC complex may play a role in lysosomes movement and localization at the cell periphery. It may indirectly play a role in cell spreading and motility. LOH12CR1 has two isoforms with MW 17 kDa and 22 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
| WB protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
| IF protocol for LOH12CR1 antibody 17169-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LOH12CR1 Polyclonal antibody (Cat# 17169-1-AP) has 8 total citations. These appear in journals including Nature Neuroscience, Autophagy, Developmental Cell, Cell Genomics, Cell Reports, American Journal of Human Genetics, and medRxiv. The associated research fields span lysosome biology and trafficking, axonal mitochondrial homeostasis, autophagy, HIV pathogenesis, autism spectrum disorder genetics, endosome-lysosome tethering, and a hypomyelinating leukodystrophy caused by BLOC1S1 variants.
| Species | Application | Title |
|---|---|---|
Nat Neurosci Messenger RNA transport on lysosomal vesicles maintains axonal mitochondrial homeostasis and prevents axonal degeneration | ||
Autophagy HIV-1 Vpr protein impairs lysosome clearance causing SNCA/alpha-synuclein accumulation in neurons. | ||
Cell Genom Protein interaction studies in human induced neurons indicate convergent biology underlying autism spectrum disorders | ||
Cell Rep Synaptic Vesicle Precursors and Lysosomes Are Transported by Different Mechanisms in the Axon of Mammalian Neurons.
| ||
Cell Rep Consecutive functions of small GTPases guide HOPS-mediated tethering of late endosomes and lysosomes |
