LPIN1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LPIN1 Polyclonal Antibody for IHC, IP, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Publications

5

Reviews (1)

Cat no : 27026-1-AP

Synonyms

KIAA0188, lipin 1, Lipin1, LPIN1, PAP1

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Validation Data Gallery

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  WB analysis using 27026-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 27026-1-AP (LPIN1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IP experiment of LNCaP using 27026-1-AP

  IP result of anti-LPIN1 (IP:27026-1-AP, 4ug; Detection:27026-1-AP 1:500) with LNCaP cells lysate 2400 ug.

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  IHC staining of human liver cancer using 27026-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27026-1-AP (LPIN1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 27026-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 27026-1-AP (LPIN1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: A549 cells, LNCaP cells
• Positive IP detected in: LNCaP cells
• Positive IHC detected in: human liver cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:8000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 5 publications below

Product Information

27026-1-AP targets LPIN1 in WB, IP, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LPIN1 fusion protein Ag25815
• Full Name: lipin 1
• Calculated Molecular Weight: 890 aa, 99 kDa
• Observed Molecular Weight: 110-130 kDa
• GenBank Accession Number: BC030537
• Gene Symbol: LPIN1
• Gene ID (NCBI): 23175
• RRID: AB_2880727
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
human	WB	Dev Cell; Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.; Authors - Holly Merta ; KD Validated
human,mouse	WB	J Agric Food Chem; Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.; Authors - Shuhua Shan
mouse	WB	Cell Mol Life Sci; Interference of a mammalian circRNA regulates lipid metabolism reprogramming by targeting miR-24-3p/Igf2/PI3K-AKT-mTOR and Igf2bp2/Ucp1 axis; Authors - Jiahao Shao
human	WB	bioRxiv; Differential reliance of CTD-nuclear envelope phosphatase 1 on its regulatory subunit in ER lipid synthesis and storage; Authors - Jake W Carrasquillo Rodríguez
mouse	WB	Reprod Toxicol; Increased NAD+ levels protect female mouse reproductive system against zearalenone-impaired glycolysis, lipid metabolism, antioxidant capacity and inflammation; Authors - Dai Chao

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Laure (Verified Customer) (07-21-2022)

this antibody recognize one protein around 130kDa in human myoblasts and no band is observed in lipin1 patient's myoblasts revelation with femto at 60sec

• Applications: Western Blot
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: human myoblasts