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LPIN1 Polyclonal antibody
LPIN1 Polyclonal Antibody for WB, IP, IHC, ELISA
Host / Isotype
Rabbit / IgG
Reactivity
human and More (2)
Applications
WB, IHC, IP, ELISA
Conjugate
Unconjugated
Cat no : 27026-1-AP
Synonyms
Validation Data Gallery
Tested Applications
Positive WB detected in | A549 cells, LNCaP cells |
Positive IP detected in | LNCaP cells |
Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
Application | Dilution |
---|---|
Western Blot (WB) | WB : 1:2000-1:8000 |
Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
KD/KO | See 1 publications below |
WB | See 7 publications below |
Product Information
27026-1-AP targets LPIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
Tested Reactivity | human |
Cited Reactivity | human, mouse, pig |
Host / Isotype | Rabbit / IgG |
Class | Polyclonal |
Type | Antibody |
Immunogen | LPIN1 fusion protein Ag25815 |
Full Name | lipin 1 |
Calculated Molecular Weight | 890 aa, 99 kDa |
Observed Molecular Weight | 110-130 kDa |
GenBank Accession Number | BC030537 |
Gene Symbol | LPIN1 |
Gene ID (NCBI) | 23175 |
RRID | AB_2880727 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Antigen affinity purification |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
Product Specific Protocols | |
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WB protocol for LPIN1 antibody 27026-1-AP | Download protocol |
IHC protocol for LPIN1 antibody 27026-1-AP | Download protocol |
IP protocol for LPIN1 antibody 27026-1-AP | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Dev Cell Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.
| ||
J Agric Food Chem Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism. | ||
Adipocyte Effects of miR-29c on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stromal cells | ||
Toxins (Basel) Combining Multiple Omics with Molecular Dynamics Reveals SCP2-Mediated Cytotoxicity Effects of Aflatoxin B1 in SW480 Cells | ||
Cell Mol Life Sci Interference of a mammalian circRNA regulates lipid metabolism reprogramming by targeting miR-24-3p/Igf2/PI3K-AKT-mTOR and Igf2bp2/Ucp1 axis | ||
bioRxiv Differential reliance of CTD-nuclear envelope phosphatase 1 on its regulatory subunit in ER lipid synthesis and storage |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Laure (Verified Customer) (07-21-2022) | this antibody recognize one protein around 130kDa in human myoblasts and no band is observed in lipin1 patient's myoblasts revelation with femto at 60sec
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