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  • KD/KO Validated

LPIN1 Polyclonal antibody

LPIN1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (2)

Applications

WB, IHC, IP, ELISA

Conjugate

Unconjugated

Cat no : 27026-1-AP

Synonyms

KIAA0188, lipin 1, Lipin1, LPIN1, PAP1



Tested Applications

Positive WB detected inA549 cells, LNCaP cells
Positive IP detected inLNCaP cells
Positive IHC detected inhuman liver cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:8000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:250-1:1000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

27026-1-AP targets LPIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.

Tested Reactivity human
Cited Reactivityhuman, mouse, pig
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LPIN1 fusion protein Ag25815
Full Name lipin 1
Calculated Molecular Weight 890 aa, 99 kDa
Observed Molecular Weight 110-130 kDa
GenBank Accession NumberBC030537
Gene Symbol LPIN1
Gene ID (NCBI) 23175
RRIDAB_2880727
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for LPIN1 antibody 27026-1-APDownload protocol
IHC protocol for LPIN1 antibody 27026-1-APDownload protocol
IP protocol for LPIN1 antibody 27026-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanWB

Dev Cell

Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.

Authors - Holly Merta
  • KD Validated
human,mouseWB

J Agric Food Chem

Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism.

Authors - Shuhua Shan
pigWB

Adipocyte

Effects of miR-29c on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stromal cells

Authors - Anjing Zhang
humanWB

Toxins (Basel)

Combining Multiple Omics with Molecular Dynamics Reveals SCP2-Mediated Cytotoxicity Effects of Aflatoxin B1 in SW480 Cells

Authors - Mengting Chen
mouseWB

Cell Mol Life Sci

Interference of a mammalian circRNA regulates lipid metabolism reprogramming by targeting miR-24-3p/Igf2/PI3K-AKT-mTOR and Igf2bp2/Ucp1 axis

Authors - Jiahao Shao
humanWB

bioRxiv

Differential reliance of CTD-nuclear envelope phosphatase 1 on its regulatory subunit in ER lipid synthesis and storage

Authors - Jake W Carrasquillo Rodríguez

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Laure (Verified Customer) (07-21-2022)

this antibody recognize one protein around 130kDa in human myoblasts and no band is observed in lipin1 patient's myoblasts revelation with femto at 60sec

  • Applications: Western Blot
  • Primary Antibody Dilution: 1/1000
  • Cell Tissue Type: human myoblasts