Tested Applications
| Positive WB detected in | A549 cells, LNCaP cells |
| Positive IP detected in | LNCaP cells |
| Positive IHC detected in | human liver cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:2000-1:8000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| KD/KO | See 1 publications below |
| WB | See 7 publications below |
Product Information
27026-1-AP targets LPIN1 in WB, IHC, IP, ELISA applications and shows reactivity with human samples.
| Tested Reactivity | human |
| Cited Reactivity | human, mouse, pig |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag25815 Product name: Recombinant human LPIN1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 301-460 aa of BC030537 Sequence: ICDKSHFQAIHSESSDTFSDQSPTLVGGALLDQNKPQTEMQFVNEEDLETLGAAAPLLPMIEELKPPSASVVQTANKTDSPSRKRDKRSRHLGADGVYLDDLTDMDPEVAALYFPKNGDPSGLAKHASDNGARSANQSPQSVGSSGVDSGVESTSDGLRD Predict reactive species |
| Full Name | lipin 1 |
| Calculated Molecular Weight | 890 aa, 99 kDa |
| Observed Molecular Weight | 110-130 kDa |
| GenBank Accession Number | BC030537 |
| Gene Symbol | LPIN1 |
| Gene ID (NCBI) | 23175 |
| RRID | AB_2880727 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q14693 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LPIN1 antibody 27026-1-AP | Download protocol |
| IP protocol for LPIN1 antibody 27026-1-AP | Download protocol |
| WB protocol for LPIN1 antibody 27026-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Dev Cell Cell cycle regulation of ER membrane biogenesis protects against chromosome missegregation.
| ||
J Agric Food Chem Inhibitory Effects of Peroxidase from Foxtail Millet Bran on Colitis-Associated Colorectal Carcinogenesis by the Blockage of Glycerophospholipid Metabolism. | ||
Adipocyte Effects of miR-29c on proliferation and adipogenic differentiation of porcine bone marrow mesenchymal stromal cells | ||
Toxins (Basel) Combining Multiple Omics with Molecular Dynamics Reveals SCP2-Mediated Cytotoxicity Effects of Aflatoxin B1 in SW480 Cells | ||
Mol Biol Cell Differential reliance of CTD-nuclear envelope phosphatase 1 on its regulatory subunit in ER lipid synthesis and storage | ||
Cell Mol Life Sci Interference of a mammalian circRNA regulates lipid metabolism reprogramming by targeting miR-24-3p/Igf2/PI3K-AKT-mTOR and Igf2bp2/Ucp1 axis |
Reviews
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
FH Laure (Verified Customer) (07-21-2022) | this antibody recognize one protein around 130kDa in human myoblasts and no band is observed in lipin1 patient's myoblasts revelation with femto at 60sec
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