LPP Recombinant monoclonal antibody

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  WB analysis using 86117-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86117-3-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86117-3-RR (LPP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 86117-3-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using LPP antibody (86117-3-RR, Clone: 250773C9 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 86117-3-RR

  Biolayer interferometry (BLl) kinetic assays of 86117-3-RR against Human LPP were performed. The affinity constant is 1.18 nM.

LPP Uni-rAb^® Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86117-3-RR

Clone No.250773C9

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, ELISA

Synonyms

LIM domain-containing preferred translocation partner in lipoma, Lipoma preferred partner, Lipoma-preferred partner

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Tested Applications

• Positive WB detected in: HeLa cells, mouse colon tissue, HEK-293 cells, MCF-7 cells, rat colon tissue
• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86117-3-RR targets LPP in WB, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag19205

  Product name: Recombinant human LPP protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-350 aa of BC130584

  Sequence: MSHPSWLPPKSTGEPLGHVPARMETTHSFGNPSISVSTQQPPKKFAPVVAPKPKYNPYKQPGGEGDFLPPPPPPLDDSSALPSISGNFPPPPPLDEEAFKVQGNPGGKTLEERRSSLDAEIDSLTSILADLECSSPYKPRPPQSSTGSTASPPVSTPVTGHKRMVIPNQPPLTATKKSTLKPQPAPQAGPIPVAPIGTLKPQPQPVPASYTTASTSSRPTFNVQVKSAQPSPHYMAAPSSGQIYGSGPQGYNTQPVPVSGQCPPPSTRGGMDYAYIPPPGLQPEPGYGYAPNQGRYYEGYYAAGPGYGGRNDSDPTYGQQGHPNTWKREPGYTPPGAGNQNPPGMYPVTG

• Full Name: LIM domain containing preferred translocation partner in lipoma
• Calculated Molecular Weight: 612 aa, 66 kDa
• Observed Molecular Weight: 70-75 kDa
• GenBank Accession Number: BC130584
• Gene Symbol: LPP
• Gene ID (NCBI): 4026
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q93052
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).