Tested Applications
Positive WB detected in | HeLa cells, mouse colon tissue, HEK-293 cells, MCF-7 cells, rat colon tissue |
Recommended dilution
Application | Dilution |
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Western Blot (WB) | WB : 1:5000-1:50000 |
It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Product Information
86117-3-RR targets LPP in WB, ELISA applications and shows reactivity with human, mouse, rat samples.
Tested Reactivity | human, mouse, rat |
Host / Isotype | Rabbit / IgG |
Class | Recombinant |
Type | Antibody |
Immunogen | LPP fusion protein Ag19205 Predict reactive species |
Full Name | LIM domain containing preferred translocation partner in lipoma |
Calculated Molecular Weight | 612 aa, 66 kDa |
Observed Molecular Weight | 70-75 kDa |
GenBank Accession Number | BC130584 |
Gene Symbol | LPP |
Gene ID (NCBI) | 4026 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Protein A purification |
UNIPROT ID | Q93052 |
Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LPP is a member of a subfamily of LIM domain proteins. Its characteristic is that the N-terminal region is rich in proline, and it has three C-terminal LIM domains. LPP localizes to the cell periphery in focal adhesions and may be involved in cell-cell adhesion and cell motility(PMID: 10637295). LPP is located at the junction of certain disease-related chromosomal translocations, which result in the expression of chimeric proteins that may promote tumor growth(PMID: 12581855).
Protocols
Product Specific Protocols | |
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WB protocol for LPP antibody 86117-3-RR | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |