LRCH3 Polyclonal antibody

LRCH3 Polyclonal Antibody for IF, IP, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IF,ELISA

Conjugate

Unconjugated

Cat no : 15800-1-AP

Synonyms

LRCH3

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Validation Data Gallery

• 

  WB analysis of human liver using 15800-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 15800-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 15800-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human spleen using 15800-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15800-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15800-1-AP (LRCH3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15800-1-AP

  IP result of anti-LRCH3 (IP:15800-1-AP, 3ug; Detection:15800-1-AP 1:1000) with mouse liver tissue lysate 12500ug.

• 

  IF Staining of A431 using 15800-1-AP

  Immunofluorescent analysis of A431 cells using 15800-1-AP (LRCH3 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: human liver tissue, human heart tissue, human spleen tissue, Jurkat cells, mouse lung tissue
• Positive IP detected in: mouse liver tissue
• Positive IF detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2400
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15800-1-AP targets LRCH3 in WB, IP, IF,ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRCH3 fusion protein Ag8511
• Full Name: leucine-rich repeats and calponin homology (CH) domain containing 3
• Calculated Molecular Weight: 86 kDa
• Observed Molecular Weight: 70 kDa
• GenBank Accession Number: BC007504
• Gene Symbol: LRCH3
• Gene ID (NCBI): 84859
• RRID: AB_2138461
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.