Tested Applications
| Positive WB detected in | HCT 116 cells, mouse heart tissue, HepG2 cells, mouse skeletal muscle tissue, mouse kidney tissue, mouse liver tissue, HeLa cells, rat kidney tissue, rat liver tissue, rat heart tissue, PC-12 cells, SH-SY5Y cells |
| Positive IP detected in | HeLa cells |
| Positive IHC detected in | human kidney tissue, human colon tissue, human heart tissue, mouse heart tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U2OS cells, HepG2 cells, HeLa cells, HEK-293 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
21175-1-AP targets LRPPRC in WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag15452 Product name: Recombinant human LRPPRC protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1014-1394 aa of BC050311 Sequence: LWYEDEKHSLNSSSASTTEPDFQKDILIACRLNQKKGAYDIFLNAKEQNIVFNAETYSNLIKLLMSEDYFTQAMEVKAFAETHIKGFTLNDAANSRLIITQVRRDYLKEAVTTLKTVLDQQQTPSRLAVTRVIQALAMKGDVENIEVVQKMLNGLEDSIGLSKMVFINNIALAQIKNNNIDAAIENIENMLTSENKVIEPQYFGLAYLFRKVIEEQLEPAVEKISIMAERLANQFAIYKPVTDFFLQLVDAGKVDDARALLQRCGAIAEQTPILLLFLLRNSRKQGKASTVKSVLELIPELNEKEEAYNSLMKSYVSEKDVTSAKALYEHLTAKNTKLDDLFLKRYASLLKYAGEPVPFIEPPESFEFYAQQLRKLRENSS Predict reactive species |
| Full Name | leucine-rich PPR-motif containing |
| Calculated Molecular Weight | 1394 aa, 158 kDa |
| Observed Molecular Weight | 130 kDa |
| GenBank Accession Number | BC050311 |
| Gene Symbol | LRPPRC |
| Gene ID (NCBI) | 10128 |
| RRID | AB_10733879 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | P42704 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRPPRC (also called LRP130 or GP130) is a 130-kDa RNA-binding protein of the pentatricopeptide repeat family. LRPPRC localizes primarily to the mitochondria where it binds to poly(A) mRNA. It plays a role in the translation or stability of mitochondrially encoded cytochrome c oxidase (COX) subunits. LRPPRC has also been shown to regulate nuclear gene transcription and to bind specific RNA molecules in both the nucleus and the cytoplasm. Mutations in the LRPPRC gene are associated with the French-Canadian type of Leigh syndrome.
Protocols
| Product Specific Protocols | |
|---|---|
| IF protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| IHC protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| IP protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| WB protocol for LRPPRC antibody 21175-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LRPPRC Polyclonal antibody (Cat# 21175-1-AP) has 58 citations published in journals including Cell, Nature Biotechnology, Nature Communications, PNAS, EMBO Journal, Science Advances, and Developmental Cell. Research fields span RNA metabolism, m6A modification, mitochondrial biology, cancer (bladder, breast, ovarian, colorectal, lung), autophagy, neurodegeneration, cardiovascular disease, and immune regulation. Cited applications include WB, IHC, IF, IP, CoIP, RIP, and ELISA across human, mouse, and rat species.
| Species | Application | Title |
|---|---|---|
Cell QKI shuttles internal m7G-modified transcripts into stress granules and modulates mRNA metabolism | ||
Nat Biotechnol Translation efficiency of mRNAs is increased by antisense oligonucleotides targeting upstream open reading frames. | ||
Autophagy A/(H1N1) pdm09 NS1 promotes viral replication by enhancing autophagy through hijacking the IAV negative regulatory factor LRPPRC | ||
Nat Commun Targeted therapies of inflammatory diseases with intracellularly gelated macrophages in mice and rats | ||
Nat Commun Mitochondrial membrane proteins and VPS35 orchestrate selective removal of mtDNA | ||
Reviews
What customers say
Both reviewers used this antibody for Western Blot and reported clean, specific bands in the expected 120–150 kDa range. One user achieved a clear ~130 kDa band in fibroblasts at a 1:1000 dilution, while another obtained a crisp band between 120–150 kDa in HEK293 cells at 1:6000 using 7% Tris-Acetate SDS-PAGE with 20 µg whole cell lysate. Overall, the antibody demonstrated reliable performance across different cell types and dilutions, earning high ratings for specificity and band clarity.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Manon (Verified Customer) (09-25-2025) | Clear band around 130 kDa
|
Angela (Verified Customer) (10-09-2018) | Worked well with 20 ug whole cell lysate run on 7% Tris-Acetate SDS-PAGE. Clean band seen between 120-150 kDa.
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