LRRC41 Polyclonal antibody

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  WB analysis using 20457-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20457-1-AP (LRRC41 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 20457-1-AP

  IP result of anti-LRRC41 (IP:20457-1-AP, 4ug; Detection:20457-1-AP 1:300) with K-562 cells lysate 1820 ug.

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  IHC staining of human testis using 20457-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 20457-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 20457-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 20457-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 20457-1-AP (LRRC41 antibody) at dilution of 1:50 (under 40x lens).

LRRC41 Polyclonal Antibody for WB, IHC, IP, ELISA

Cat No. 20457-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IP, ELISA

Synonyms

Protein Muf1, PP7759, MUF1, Leucine-rich repeat-containing protein 41

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Tested Applications

• Positive WB detected in: H9C2 cells
• Positive IP detected in: K-562 cells
• Positive IHC detected in: human testis tissue, human liver tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

20457-1-AP targets LRRC41 in WB, IHC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14266

  Product name: Recombinant human LRRC41 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 223-572 aa of BC004953

  Sequence: STLELFTVPLSTEAALTLCHLLSSWVSLESLTLSYNGLGSNIFRLLDSLRALSGQAGCRLRALHLSDLFSPLPILELTRAIVRALPLLRVLSIRVDHPSQRDNPGVPGNAGPPSHIIGDEEIPENCLEQLEMGFPRGAQPAPLLCSVLKASGSLQQLSLDSATFASPQDFGLVLQTLKEYNLALKRLSFHDMNLADCQSEVLFLLQNLTLQEITFSFCRLFEKRPAQFLPEMVAAMKGNSTLKGLRLPGNRLGNAGLLALADVFSEDSSSSLCQLDISSNCIKPDGLLEFAKRLERWGRGAFGHLRLFQNWLDQDAVTAREAIRRLRATCHVVSDSWDSSQAFADYVSTM

• Full Name: leucine rich repeat containing 41
• Calculated Molecular Weight: 812 aa, 87 kDa
• Observed Molecular Weight: 80-90 kDa
• GenBank Accession Number: BC004953
• Gene Symbol: LRRC41
• Gene ID (NCBI): 10489
• RRID: AB_10858385
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q15345
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.