Tested Applications
| Positive WB detected in | rat lung tissue |
| Positive IP detected in | mouse brain tissue |
| Positive IHC detected in | human colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunoprecipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Published Applications
| WB | See 1 publications below |
| IHC | See 1 publications below |
| IF | See 1 publications below |
Product Information
17155-1-AP targets LRRC8A/SWELL1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10062 Product name: Recombinant human LRRC8A protein Source: e coli.-derived, PET28a Tag: 6*His Domain: 464-810 aa of BC051322 Sequence: SIAQLTGLKELWLYHTAAKIEAPALAFLRENLRALHIKFTDIKEIPLWIYSLKTLEELHLTGNLSAENNRYIVIDGLRELKRLKVLRLKSNLSKLPQVVTDVGVHLQKLSINNEGTKLIVLNSLKKMANLTELELIRCDLERIPHSIFSLHNLQEIDLKDNNLKTIEEIISFQHLHRLTCLKLWYNHIAYIPIQIGNLTNLERLYLNRNKIEKIPTQLFYCRKLRYLDLSHNNLTFLPADIGLLQNLQNLAITANRIETLPPELFQCRKLRALHLGNNVLQSLPSRVGELTNLTQIELRGNRLECLPVELGECPLLKRSGLVVEEDLFNTLPPEVKERLWRADKEQA Predict reactive species |
| Full Name | leucine rich repeat containing 8 family, member A |
| Calculated Molecular Weight | 810 aa, 94 kDa |
| Observed Molecular Weight | 94 kDa |
| GenBank Accession Number | BC051322 |
| Gene Symbol | LRRC8A |
| Gene ID (NCBI) | 56262 |
| RRID | AB_2918050 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q8IWT6 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LRRC8A, also named as SWELL1, KIAA1437 and LRRC8, belongs to the LRRC8 family. LRRC8A is a ubiquitous and integral component of the VRAC and has been reported to be required for regulation of cell volume. It indicates that LRRC8A plays a role in regulating the flow of ions, especially anions, into and out of cells (PMID: 32079488). Additionally, there have been several reports that LRRC8A is critical for differentiation of adipocytes and lymphocytes (PMID: 31323188). The molecular weight of LRRC8A is 94 kDa.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LRRC8A/SWELL1 antibody 17155-1-AP | Download protocol |
| IP protocol for LRRC8A/SWELL1 antibody 17155-1-AP | Download protocol |
| WB protocol for LRRC8A/SWELL1 antibody 17155-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Int J Biol Sci Upregulation of LRRC8A by m5C modification-mediated mRNA stability suppresses apoptosis and facilitates tumorigenesis in cervical cancer | ||
Inflammation NOX4 Regulates NLRP3 by Inhibiting the Ubiquitination of LRRC8A to Promote Ferroptosis in Nucleus Pulposus Cells |
