LRRC8C Polyclonal antibody

LRRC8C Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

1

Reviews (1)

Cat no : 21601-1-AP

Synonyms

AD158, FAD158, LRRC8C

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Validation Data Gallery

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  WB analysis using 21601-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 21601-1-AP (LRRC8C antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 21601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21601-1-AP (LRRC8C antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 21601-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 21601-1-AP (LRRC8C antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 21601-1-AP

  Immunofluorescent analysis of HepG2 cells, using LRRC8C antibody 21601-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Tested Applications

• Positive WB detected in: THP-1 cells
• Positive IHC detected in: mouse heart tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

Sample-dependent, check data in validation data gallery

Published Applications

• WB: See 1 publications below
• IF: See 1 publications below

Product Information

The immunogen of 21601-1-AP is LRRC8C Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LRRC8C fusion protein Ag16213
• Full Name: leucine rich repeat containing 8 family, member C
• Calculated molecular weight: 803 aa, 92 kDa
• Observed molecular weight: 92 kDa
• GenBank accession number: BC113973
• Gene symbol: LRRC8C
• Gene ID (NCBI): 84230
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	WB,IF	Biomedicines; Astrocytes-Derived Small Extracellular Vesicles Hinder Glioma Growth; Authors - Carmela Serpe

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Joshua (Verified Customer) (08-16-2021)

This antibody was incubated at 4 degrees C O/N, in 5% Milk, and at a dilution ratio of 1:1,000. A secondary antibody at a dilution ratio of 1:5,000 was used. The acquired western blot image was achieved at an exposure time of 60 seconds. This product was vital in visualizing LRRC8c in both HUVECs and mouse lung tissue.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1,000
• Cell Tissue Type: HUVECs and Mouse Lung Tissue