LRRFIP1 Recombinant monoclonal antibody

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  WB analysis using 84778-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84778-2-RR (LRRFIP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  Affinity and Kinetic Characterization of 84778-2-RR

  Biolayer interferometry (BLl) kinetic assays of 84778-2-RR against Human LRRFIP1 were performed. The affinity constant is below 1 pM.

LRRFIP1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 84778-2-RR

Clone No.242161F10

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, ELISA

Synonyms

GCF 2, GC-binding factor 2, GC binding factor 2, FLIIAP1, FLAP 1

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Tested Applications

• Positive WB detected in: A549 cells, Raji cells, HEK-293 cells, COLO 320 cells, HL-60 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84778-2-RR targets LRRFIP1 in WB, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag34760

  Product name: Recombinant human LRRFIP1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 500-808 aa of BC108914

  Sequence: EEEGEETGLRDEKPIKTEVPGSPAGTEGNCQEATGPSTVDTQNEPLDMKEPDEEKSDQQGEAFDSSQKKTKNKKKKNKKKKSPVPVETLKDVKKELTYQNTDLSEIKEEEQVKSTDRKSAVEAQNEVTENPKQKIAAESSENVDCPENPKIKLDGKLDQEGDDVQTAAEEVLADGDTLDFEDDTVQSSGPRAGGEELDEGVAKDNAKIDGATQSSPAEPKSEDADRCTLPEHESPSQDISDACEAESTERCEMSEHPSQTVRKALDSNSLENDDLSAPGREPGHFNPESREDTRGGNEKGKSKEDCTMS

• Full Name: leucine rich repeat (in FLII) interacting protein 1
• Observed Molecular Weight: 150-170 kDa
• GenBank Accession Number: BC108914
• Gene Symbol: LRRFIP1
• Gene ID (NCBI): 9208
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q32MZ4
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LRRFIP1 is a transcriptional repressor which preferentially binds to the GC-rich consensus sequence (5'-AGCCCCCGGCG-3') and may regulate expression of TNF, EGFR and PDGFA. The endogenous LRRFIP1/GCF2 proteins in several human cell lines, the one expressed from LRRFIP1/GCF2 cDNA in reticulolysates, and the recombinant LRRFIP1/GCF2 protein produced in bacteria have migrated as a band with a molecular weight (MW) of 160 kda in sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE).