LRSAM1 Polyclonal antibody, PBS Only

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• LRSAM1 Polyclonal antibody, PBS Only (24666-1-PBS)

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  WB analysis of Jurkat using 24666-1-AP (same clone as 24666-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse liver using 24666-1-AP (same clone as 24666-1-PBS)

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  WB analysis of HepG2 using 24666-1-AP (same clone as 24666-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 24666-1-AP (LRSAM1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  IHC staining of human skeletal muscle using 24666-1-AP (same clone as 24666-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle slide using 24666-1-AP (LRSAM1 Antibody) at dilution of 1:50. This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 24666-1-AP (same clone as 24666-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using LRSAM1 antibody (24666-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2). This data was developed using the same antibody clone with 24666-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

LRSAM1 Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Cat No. 24666-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF/ICC, Indirect ELISA

Synonyms

RING-type E3 ubiquitin transferase LRSAM1, RIFLE, Leucine-rich repeat and sterile alpha motif-containing protein 1, hTAL, EC:2.3.2.27

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Product Information

24666-1-PBS targets LRSAM1 in WB, IHC, IF/ICC, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag20415

  Product name: Recombinant human LRSAM1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 501-723 aa of BC009239

  Sequence: QEMISEQRWALSSLLQQLLKEKQQREEELREILTELEAKSETRQENYWLIQYQRLLNQKPLSLKLQEEGMERQLVALLEELSAEHYLPIFAHHRLSLDLLSQMSPGDLAKVGVSEAGLQHEILRRVQELLDAARIQPELKPPMGEVVTPTAPQEPPESVRPSAPPAELEVQASECVVCLEREAQMIFLNCGHVCCCQQCCQPLRTCPLCRQDIAQRLRIYHSS

• Full Name: leucine rich repeat and sterile alpha motif containing 1
• Calculated Molecular Weight: 723 aa, 84 kDa
• Observed Molecular Weight: 84 kDa
• GenBank Accession Number: BC009239
• Gene Symbol: LRSAM1
• Gene ID (NCBI): 90678
• RRID: AB_2879665
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q6UWE0
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

LRSAM1 also named as hTAL, RIFLE orTsg101 associated ligase is a 723 amino acid protein, which contains 6 LRR repeats, 1 Ring-type zinc finger and 1 SAM domain. LRSAM1 is localizes in cytoplasm and highly expressed in adult spinal cord motoneurons as well as in fetal spinal cord and muscle tissue. As a E3 ubiquitin protein ligase, LRSAM1 mediates monoubiquitination of TSG101 at multiple sites, leading to inactivate the ability of TSG101 to sort endocytic and exocytic cargos, LRSAM1 gene mutation has an association with Charcot Maire Tooth disease 2P.