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LSM1 Polyclonal antibody

LSM1 Polyclonal Antibody for WB, IP, IHC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IP, IHC, ELISA

Conjugate

Unconjugated

Cat no : 10259-1-AP

Synonyms

Cancer associated Sm like, CASM, LSM1, Small nuclear ribonuclear CaSm, YJL124C

Validation Data Gallery

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  • Western Blot (WB) analysis of HeLa cells using LSM1 Polyclonal antibody (10259-1-AP)

    WB analysis of HeLa using 10259-1-AP

    HeLa cells were subjected to SDS PAGE followed by western blot with 10259-1-AP (LSM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Western Blot (WB) analysis of mouse kidney tissue using LSM1 Polyclonal antibody (10259-1-AP)

    WB analysis of mouse kidney using 10259-1-AP

    mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10259-1-AP (LSM1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

  • Immunoprecipitation (IP) experiment of HeLa cells using LSM1 Polyclonal antibody (10259-1-AP)

    IP experiment of HeLa using 10259-1-AP

    IP result of anti-LSM1 (IP:10259-1-AP, 4ug; Detection:10259-1-AP 1:300) with HeLa cells lysate 2000ug.

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using LSM1 Polyclonal antibody (10259-1-AP)

    IHC staining of human prostate cancer using 10259-1-AP

    Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10259-1-AP (LSM1 antibody) at dilution of 1:100 (under 10x lens).

Tested Applications

Positive WB detected inHeLa cells, mouse kidney tissue
Positive IP detected inHeLa cells
Positive IHC detected inhuman prostate cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunoprecipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochemistry (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

10259-1-AP targets LSM1 in WB, IP, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LSM1 fusion protein Ag0232
Full Name LSM1 homolog, U6 small nuclear RNA associated (S. cerevisiae)
Calculated Molecular Weight 15 kDa
Observed Molecular Weight 18-20 kDa
GenBank Accession NumberBC001767
Gene Symbol LSM1
Gene ID (NCBI) 27257
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lsm1 is a component of the Lsm1-7 complex involved in cytoplasmic mRNA degradation. Lsm1 is a key subunit that determines the RNA-binding properties of this complex. The normal RNA-binding activity of this complex is crucial for mRNA decay and 3'-end protection in vivo and requires the intact Sm-domain of Lsm1. LSM1 localizes to cytoplasmic processing bodies (P bodies) involved in mRNA storage, processing, regulation, and degradation and is required for P-body formation. LSM1 proteins facilitate the formation of U4/U6 RNA duplexes in vitro and may play a role in U4/U6 snRNP formation.