LSM14A Polyclonal antibody

LSM14A Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human and More (1)

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugate

Unconjugated

Publications

13

Cat no : 18336-1-AP

Synonyms

Protein FAM61A, hRAP55A, hRAP55, FAM61A, C19orf13

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Validation Data Gallery

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  WB analysis using 18336-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 18336-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 18336-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 18336-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 18336-1-AP (LSM14A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 18336-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18336-1-AP (LSM14A antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 18336-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 18336-1-AP (LSM14A antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HepG2 using 18336-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 18336-1-AP (LSM14A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Tested Applications

• Positive WB detected in: A549 cells, HeLa cells, PC-3 cells, HEK-293T cells, HepG2 cells, Jurkat cells
• Positive IHC detected in: human testis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IF: See 8 publications below
• CoIP: See 1 publications below

Product Information

18336-1-AP targets LSM14A in WB, IHC, IF/ICC, CoIP, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LSM14A fusion protein Ag12997
• Full Name: LSM14A, SCD6 homolog A (S. cerevisiae)
• Calculated Molecular Weight: 463 aa, 51 kDa
• Observed Molecular Weight: 60 kDa
• GenBank Accession Number: BC016842
• Gene Symbol: LSM14A
• Gene ID (NCBI): 26065
• RRID: AB_10644339
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LSm14A was originally discovered to be a component of the P-bodies, it is a member of the LSm family of proteins, which have been shown to be involved in RNA processing events. The N terminus of LSm14A is a conserved LSm domain, whereas the C terminus contains two domains called "DFDF box" and "FDF_TFG box", respectively.

Publications

Species	Application	Title
human	IF	Cancer Res; Pooled CRISPR Screening Identifies P-Bodies as Repressors of Cancer Epithelial-Mesenchymal Transition; Authors - Liang Fang
human	WB,IF	Elife; Selective sorting of microRNAs into exosomes by phase-separated YBX1 condensates.; Authors - Xiao-Man Liu
mouse	IF	Cell Rep; The proteome and transcriptome of stress granules and P bodies during human T lymphocyte activation; Authors - Nicolas Curdy
human	IF	Adv Sci (Weinh); Oleic Acid Dissolves cGAS-DNA Phase Separation to Inhibit Immune Surveillance; Authors - Lina Wang
human	IF	Nucleic Acids Res; RNA helicase DDX6 and scaffold protein GW182 in P-bodies promote biogenesis of stress granules; Authors - Vladimir Majerciak
human	IF,WB	J Cell Biol; DDX6 modulates P-body and stress granule assembly, composition, and docking; Authors - Nina Ripin