LSR Monoclonal antibody

LSR Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1G9B7

Cat no : 67508-1-Ig

Synonyms

ILDR3, LISCH, LISCH7, LSR

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Validation Data Gallery

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  WB analysis of LNCaP using 67508-1-Ig

  LNCaP cells were subjected to SDS PAGE followed by western blot with 67508-1-Ig (LSR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67508-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67508-1-Ig (LSR antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: LNCaP cells, MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67508-1-Ig targets LSR in WB, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: LSR fusion protein Ag13205
• Full Name: lipolysis stimulated lipoprotein receptor
• Calculated Molecular Weight: 71 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC004381
• Gene Symbol: LSR
• Gene ID (NCBI): 51599
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LSR (lipolysis stimulated lipoprotein receptor) is a lipoprotein receptor primarily expressed in the liver and activated by free fatty acids (PMID: 10224102). This remnant receptor binds both apoB- and apoE-containing lipoproteins and displays greatest affinity for TG-rich lipoproteins. Distribution of LSR causes embryonic lethality in mice, indicating that the expression of LSR is critical for liver and embryonic development (PMID: 15265030). Investigations show that LSR is a multimer consisting of α subunit (68 kDa) and β subunit (56 kDa) associated through disulfide bridges (PMID: 10224102; 19188430; 18644789).