LTBR Polyclonal antibody

• Featured Product
• KD/KO Validated

LTBR Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, FC, ELISA

Conjugate

Unconjugated

Publications

6

Cat no : 20331-1-AP

Synonyms

D12S370, LT BETA R, LTBR, Lymphotoxin beta receptor, TNF R III, TNF RIII, TNFCR, TNFR III, TNFR RP, TNFR2 RP, TNFR3, TNFRSF3

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Validation Data Gallery

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  WB analysis of HeLa using 20331-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20331-1-AP (LTBR antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20331-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20331-1-AP (LTBR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20331-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20331-1-AP (LTBR antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 20331-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20331-1-AP (LTBR antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human hepatocirrhosis using 20331-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20331-1-AP (LTBR antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hepatocirrhosis using 20331-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20331-1-AP (LTBR antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hepatocirrhosis using 20331-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20331-1-AP (LTBR antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human hepatocirrhosis using 20331-1-AP

  Immunohistochemical analysis of paraffin-embedded human hepatocirrhosis using 20331-1-AP (LTBR antibody) at dilution of 1:50 (under 40x lens).

• 

  FC experiment of HEK-293 using 20331-1-AP

  1X10^6 HEK-293 cells were stained with 0.2ug LTBR antibody (20331-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Tested Applications

• Positive WB detected in: HeLa cells, mouse brain tissue
• Positive IHC detected in: human hepatocirrhosis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: HEK-293 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:5000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 4 publications below

Product Information

The immunogen of 20331-1-AP is LTBR Fusion Protein expressed in E. coli.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LTBR fusion protein Ag14065
• Full Name: lymphotoxin beta receptor (TNFR superfamily, member 3)
• Calculated Molecular Weight: 435 aa, 47 kDa
• Observed Molecular Weight: 47-60 kDa
• GenBank Accession Number: BC026262
• Gene Symbol: LTBR
• Gene ID (NCBI): 4055
• RRID: AB_10694276
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

Species	Application	Title
mouse	IF	Mol Ther; Activation of NF-κB-Inducing Kinase in Islet β Cells Causes β Cell Failure and Diabetes.; Authors - Xinzhi Li
human	WB	J Immunol; SNX17 Affects T Cell Activation by Regulating TCR and Integrin Recycling.; Authors - Douglas G Osborne
human	IHC,IF,WB	Am J Pathol; Lymphotoxins Promote the Progression of Human Lymphatic Malformation by Enhancing Lymphatic Endothelial Cell Proliferation.; Authors - Jie-Gang Yang ; KD Validated
		Mol Med Rep; Lymphotoxin β receptor activation promotes mRNA expression of RelA and pro-inflammatory cytokines TNFα and IL-1β in bladder cancer cells.; Authors - Mo Shen
mouse	IF	Theranostics; Islet α-cell Inflammation Induced By NF-κB inducing kinase (NIK) Leads to Hypoglycemia, Pancreatitis, Growth Retardation, and Postnatal Death in Mice.; Authors - Xinzhi Li
human	IHC,IF	J Reprod Immunol; A LIGHT-HVEM/LTβR axis contributes to the fibrosis of intrauterine adhesion; Authors - Ayitila Abudukeyoumu