CoraLite® Plus 488-conjugated LUC7L Recombinant monoclonal antibody

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  IF Staining of HeLa using CL488-83585

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 488 LUC7L antibody (CL488-83585, Clone: 240311B10 ) at dilution of 1:200, CL594-phalloidin (red).

• Featured Product
• KD/KO Validated

LUC7L Uni-rAb^® Recombinant Antibody for IF/ICC

Cat No. CL488-83585

Clone No.240311B10

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

IF/ICC

Synonyms

hLuc7B1, LUC7 LIKE, LUC7B1, LUC7L1, Putative RNA-binding protein Luc7-like 1

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Tested Applications

• Positive IF/ICC detected in: HeLa cells

Recommended dilution

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-83585 targets LUC7L in IF/ICC applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag9170

  Product name: Recombinant human LUC7L protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1-354 aa of BC065198

  Sequence: MQMGDETRQRVKFTDDRVCKSHLLDCCPHDILAGTRMDLGECTKIHDLALRADYEIASKERDLFFELDAMDHLESFIAECDRRTELAKKRLAETQEEISAEVSAKAEKVHELNEEIGKLLAKAEQLGAEGNVDESQKILMEVEKVRAKKKEAEEEYRNSMPASSFQQQKLRVCEVCSAYLGLHDNDRRLADHFGGKLHLGFIQIREKLDQLRKTVAEKQEKRNQDRLRRREEREREERLSRRSGSRTRDRRRSRSRDRRRRRSRSTSRERRKLSRSRSRDRHRRHRSRSRSHSRGHRRASRDRSAKYKFSRERASREESWESGRSERGPPDWRLESSNGKMASRRSEEKEAGEI

• Full Name: LUC7-like (S. cerevisiae)
• Calculated Molecular Weight: 44 kDa
• Observed Molecular Weight: 38 kDa and 44 kDa
• GenBank Accession Number: BC065198
• Gene Symbol: LUC7L
• Gene ID (NCBI): 55692
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q9NQ29
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

LUC7L, also known as LUC7L1, belongs to the Luc7 family. LUC7L encodes a putative RNA binding protein similar to the yeast Luc7p subunit of the U1 snRNP splicing complex, which is normally required for 5` splice site selection (PMID: 15474286). LUC7L is widely expressed. LUC7L has 3 isoforms with the molecular mass of 38, 42 and 44 kDa.