LUZP1 Polyclonal antibody

• Featured Product
• KD/KO Validated

LUZP1 Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

9

Cat no : 17483-1-AP

Synonyms

LUZP

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Validation Data Gallery

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  WB analysis of mouse brain using 17483-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17483-1-AP (LUZP1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis using 17483-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17483-1-AP (LUZP1 antibody) at dilution of 1:15000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 17483-1-AP

  IP result of anti-LUZP1 (IP:17483-1-AP, 4ug; Detection:17483-1-AP 1:10000) with mouse brain tissue lysate 1680 ug.

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  IHC staining of human ovary tumor using 17483-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 17483-1-AP (LUZP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 17483-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17483-1-AP (LUZP1 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 17483-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 17483-1-AP (LUZP1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Tested Applications

• Positive WB detected in: mouse brain tissue, rat brain tissue
• Positive IP detected in: mouse brain tissue
• Positive IHC detected in: human ovary tumor tissue, rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 8 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Product Information

17483-1-AP targets LUZP1 in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LUZP1 fusion protein Ag11518
• Full Name: leucine zipper protein 1
• Calculated Molecular Weight: 1026 aa, 115 kDa
• Observed Molecular Weight: 120 kDa
• GenBank Accession Number: BC051733
• Gene Symbol: LUZP1
• Gene ID (NCBI): 7798
• RRID: AB_2139498
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LUZP1, also known as LUZP, is predominantly expressed in the brain, whereas it is present in the nucleus of neurons. LUZP1 is a centrosome, actin, and midbody-localizing protein implicated in ciliogenesis regulation and actin cytoskeleton stability (PMID: 33869174). LUZP1 null mice present defective neural tube closure and cardiovascular problems, which cause perinatal death. In addition, LUZP1 has also been implicated in the etiology of diseases like the 1p36 and the Townes-Brocks syndromes (PMID: 35738212).

Publications

Species	Application	Title
human	WB	Elife; LUZP1, a novel regulator of primary cilia and the actin cytoskeleton, is a contributing factor in Townes-Brocks Syndrome.; Authors - Laura Bozal-Basterra
human	WB	Front Oncol; The Circ_0001367/miR-545-3p/LUZP1 Axis Regulates Cell Proliferation, Migration and Invasion in Glioma Cells.; Authors - Xuchen Dong
mouse	WB	J Biol Chem; Activating transcription factor 4 (ATF4) promotes skeletal muscle atrophy by forming a heterodimer with the transcriptional regulator C/EBPβ.; Authors - Scott M Ebert
		Eur J Cell Biol; LUZP1: A new player in the actin-microtubule cross-talk.; Authors - João Gonçalves
mouse	WB	Biochem Cell Biol; Chromatin remodeling factor CECR2 forms tissue-specific complexes with CCAR2 and LUZP1.; Authors - Farshad Niri
human	WB	bioRxiv; Histone chaperone HIRA, Promyelocytic Leukemia (PML) protein and p62/SQSTM1 coordinate to regulate inflammation during cell senescence; Authors - Nirmalya Dasgupta