LXN Polyclonal antibody

LXN Polyclonal Antibody for WB, IHC, IP, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, IP, ELISA

Conjugate

Unconjugated

Publications

4

Reviews (2)

Cat no : 13056-1-AP

Synonyms

TCI, Protein MUM, Latexin, ECI

---

Validation Data Gallery

• 

  WB analysis of mouse lung using 13056-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 13056-1-AP (LXN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse lung using 13056-1-AP

  IP result of anti-LXN (IP:13056-1-AP, 4ug; Detection:13056-1-AP 1:500) with mouse lung tissue lysate 5160ug.

• 

  IHC staining of human intrahepatic cholangiocarcinoma using 13056-1-AP

  Immunohistochemical analysis of paraffin-embedded human intrahepatic cholangiocarcinoma tissue slide using 13056-1-AP (LXN antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Tested Applications

• Positive WB detected in: mouse lung tissue
• Positive IP detected in: mouse lung tissue
• Positive IHC detected in: human intrahepatic cholangiocarcinoma tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IF: See 3 publications below

Product Information

13056-1-AP targets LXN in WB, IHC, IF, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LXN fusion protein Ag3726
• Full Name: latexin
• Calculated Molecular Weight: 222 aa, 26 kDa
• Observed Molecular Weight: 26 kDa
• GenBank Accession Number: BC005346
• Gene Symbol: LXN
• Gene ID (NCBI): 56925
• RRID: AB_2139511
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Latexin, also named as LTXN, LXN, ECI, MUM and TCI, is hardly reversible, non-competitive, and potent inhibitor of CPA1, CPA2 and CPA4. It may function as a neuroprotective mechanism against tau toxicity.

Publications

Species	Application	Title
mouse	WB,IF	Brain Res; Astrocytic neuroprotection through induction of cytoprotective molecules; a proteomic analysis of mutant P301S tau-transgenic mouse.; Authors - Yata Kenichiro K
mouse	WB	Cell Rep; Latexin regulates sex dimorphism in hematopoiesis via gender-specific differential expression of microRNA 98-3p and thrombospondin 1; Authors - Xiaojing Cui
mouse	IF	Nat Neurosci; Derivation and transcriptional reprogramming of border-forming wound repair astrocytes after spinal cord injury or stroke in mice; Authors - Timothy M O'Shea
mouse	WB,IF	Int J Biol Macromol; Carboxypeptidase inhibitor Latexin (LXN) regulates intestinal organogenesis and intestinal remodeling involved in intestinal injury repair in mice; Authors - Lingzhu Wang ; KD Validated

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Kyosuke (Verified Customer) (06-12-2019)

It works for IP study.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: HEK293T

FH

Kyosuke (Verified Customer) (06-05-2019)

It works for WB and IP very well.

• Applications: Western Blot, Immunoprecipitation,
• Primary Antibody Dilution: 1/1000
• Cell Tissue Type: aorta, HUVECs, RASMC, 293T