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  • KD/KO Validated

LY6D Polyclonal antibody

LY6D Polyclonal Antibody for IHC, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF,ELISA

Conjugate

Unconjugated

Cat no : 17361-1-AP

Synonyms

E48, E48 antigen, Ly 6D, LY6D, Lymphocyte antigen 6D



Tested Applications

Positive WB detected inmouse spleen tissue
Positive IHC detected inhuman tonsillitis tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:200-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Sample-dependent, check data in validation data gallery

Product Information

The immunogen of 17361-1-AP is LY6D Fusion Protein expressed in E. coli.

Tested Reactivity human, mouse
Cited Reactivity human, mouse
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen LY6D fusion protein Ag10714
Full Name lymphocyte antigen 6 complex, locus D
Calculated molecular weight 128 aa, 13 kDa
Observed molecular weight 13-14 kDa
GenBank accession numberBC031330
Gene symbol LY6D
Gene ID (NCBI) 8581
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Background Information

LY6D, also named as E48 and ThB, may act as a specification marker at earliest stage specification of lymphocytes between B- and T-cell development. It marks the earliest stage of B-cell specification.

Protocols

Product Specific Protocols
WB protocol for LY6D antibody 17361-1-APDownload protocol
IHC protocol for LY6D antibody 17361-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle

Cell Stem Cell

Single-Cell Analysis of the Liver Epithelium Reveals Dynamic Heterogeneity and an Essential Role for YAP in Homeostasis and Regeneration.

Authors - Brian J Pepe-Mooney
human,mouseIHC,IF

Cancers (Basel)

Lung Adenocarcinoma Mouse Models Based on Orthotopic Transplantation of Syngeneic Tumor-Initiating Cells Expressing EpCAM, SCA-1, and Ly6d.

Authors - Takashi Semba
  • KD Validated
mouseIF

Elife

RUNX1 marks a luminal castration-resistant lineage established at the onset of prostate development.

Authors - Renaud Mevel
mouseWB

Diabetes

PMID: 28325853

Authors - Yu-Yao Zhang
human,mouseIF

Nat Commun

AP-1 and TGFß cooperativity drives non-canonical Hedgehog signaling in resistant basal cell carcinoma.

Authors - Catherine D Yao