Tested Applications
| Positive WB detected in | HEK-293T cells, HeLa cells |
| Positive IHC detected in | human liver cancer tissue, mouse kidney tissue, human kidney tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:3000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
16146-1-AP targets LYPLAL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag9271 Product name: Recombinant human LYPLAL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-237 aa of BC016711 Sequence: MAAASGSVLQRCIVSPAGRHSASLIFLHGSGDSGQGLRMWIKQVLNQDLTFQHIKIIYPTAPPRSYTPMKGGISNVWFDRFKITNDCPEHLESIDVMCQVLTDLIDEEVKSGIKKNRILIGGFSMGGCMAMHLAYRNHQDVAGVFALSSFLNKASAVYQALQKSNGVLPELFQCHGTADELVLHSWAEETNSMLKSLGVTTKFHSFPNVYHELSKTELDILKLWILTKLPGEMEKQK Predict reactive species |
| Full Name | lysophospholipase-like 1 |
| Calculated Molecular Weight | 237 aa, 26 kDa |
| Observed Molecular Weight | 26-30 kDa |
| GenBank Accession Number | BC016711 |
| Gene Symbol | LYPLAL1 |
| Gene ID (NCBI) | 127018 |
| RRID | AB_2138521 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q5VWZ2 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Lysophospholipase-like 1 protein, encoded by LYPLAL1, a 26 kDa cytosol protein which belongs to a subclass of lysophospholipase family. Human genome-wide association studies link variants of the gene encoding this enzyme to fat distribution, waist-to-hip ratio and non-alcoholic fatty liver disease. LYPLAL1 reported to be up-regulated in subcutaneous adipose tissue of obese individuals.(PMID: 25958046, PMID: 27391855, PMID: 21674055)
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LYPLAL1 antibody 16146-1-AP | Download protocol |
| WB protocol for LYPLAL1 antibody 16146-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LYPLAL1 Polyclonal antibody (Cat# 16146-1-AP) has 4 citations published in Molecular Cell, Nature Chemical Biology, Disease Models & Mechanisms, and Journal of Molecular Endocrinology. Research fields span tumor immunotherapy (cGAS depalmitoylation), activity-based protein profiling for enzyme activator discovery, and fat deposition/obesity studies in mice. Applications cited include Western blot and IHC in human and mouse samples.
| Species | Application | Title |
|---|---|---|
Mol Cell Targeting LYPLAL1-mediated cGAS depalmitoylation enhances the response to anti-tumor immunotherapy | ||
Nat Chem Biol Discovery of small-molecule enzyme activators by activity-based protein profiling. | ||
J Mol Endocrinol Knockout of murine Lyplal1 confers sex-specific protection against diet-induced obesity |
Reviews
What customers say
The single review for 16146-1-AP is a 5-star rating from a Western Blot application. The user reported that the antibody works perfectly for detecting both overexpressed and endogenous protein in 293T cells. Overall, the feedback is highly positive, indicating reliable performance in WB.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Bernard (Verified Customer) (11-11-2019) | Works perfectly for overexpressed protein and endogenous protein in 293T cells
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