LYVE1 Polyclonal antibody

LYVE1 Polyclonal Antibody for WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Publications

7

Cat no : 51011-1-AP

Synonyms

CRSBP 1, CRSBP1, HAR, Hyaluronic acid receptor, LYVE 1, LYVE1, XLKD1

---

Validation Data Gallery

• 

  WB analysis of human brain using 51011-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 51011-1-AP (LYVE1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51011-1-AP (LYVE1 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51011-1-AP (LYVE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 51011-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 51011-1-AP (LYVE1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

---

Tested Applications

• Positive WB detected in: HUVEC cells, human brain tissue, bEnd.3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 2 publications below
• IHC: See 4 publications below
• IF: See 1 publications below

Product Information

51011-1-AP targets LYVE1 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: LYVE1 fusion protein Ag0570
• Full Name: lymphatic vessel endothelial hyaluronan receptor 1
• Calculated Molecular Weight: 322 aa, 35 kDa
• Observed Molecular Weight: 60-70 kDa
• GenBank Accession Number: BC026231
• Gene Symbol: LYVE1
• Gene ID (NCBI): 10894
• RRID: AB_2138655
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYVE1 is a 322-amino acid type-I integral membrane glycoprotein primarily expressed on lymphatic endothelial cells. LYVE1 is a CD44 homologue. It acts as a receptor and binds to both soluble and immobilized hyaluronan. LYVE1 may function in lymphatic hyaluronan transport and have a role in tumor metastasis.

Publications

Species	Application	Title
mouse	IHC	Cell Rep; Apatinib remodels the immunosuppressive tumor ecosystem of gastric cancer enhancing anti-PD-1 immunotherapy; Authors - Qicong Luo
human		Cell Death Dis; PTBP3 contributes to the metastasis of gastric cancer by mediating CAV1 alternative splicing.; Authors - Xin Liang
human,mouse	WB,IHC	Biomedicines; Heteronemin Suppresses Lymphangiogenesis through ARF-1 and MMP-9/VE-Cadherin/Vimentin.; Authors - Hsien-Lin Chen
mouse	IF	Front Physiol; Endothelial Microparticle-Mediated Transfer of microRNA-19b Inhibits the Function and Distribution of Lymphatic Vessels in Atherosclerotic Mice.; Authors - Shi-Ran Yu
mouse	IHC	Ann Transl Med; Clostridium butyricum alleviates dextran sulfate sodium-induced experimental colitis and promotes intestinal lymphatic vessel regeneration in mice.; Authors - Xing Chen
human	WB	Biol Pharm Bull; Establishment and characterization of a human lymphatic endothelial cell line.; Authors - Ryosuke Nakamura