Tested Applications
| Positive WB detected in | mouse testis tissue |
| Positive IHC detected in | human testis tissue, human lung tissue, human spleen tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:100-1:500 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
17207-1-AP targets LYZL1 in WB, IHC, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag10986 Product name: Recombinant human LYZL1 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-194 aa of BC021730 Sequence: MQDAPLSCLSPTRWSSVSSADSTEKSASGAGTRNLPFQFCLRQALRMKAAGILTLIGCLVTGAESKIYTRCKLAKIFSRAGLDNYWGFSLGNWICMAYYESGYNTTAPTVLDDGSIDYGIFQINSFAWCRRGKLKENNHCHVACSALITDDLTDAIICARKIVKETQGMNYWQGWKKHCEGRDLSEWKKGCEVS Predict reactive species |
| Full Name | lysozyme-like 1 |
| Calculated Molecular Weight | 194 aa, 22 kDa |
| Observed Molecular Weight | 22 kDa |
| GenBank Accession Number | BC021730 |
| Gene Symbol | LYZL1 |
| Gene ID (NCBI) | 84569 |
| RRID | AB_2138916 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q6UWQ5 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
LYZL1(Lysozyme-like 1) is also named as LYC2 and belongs to the glycosyl hydrolase 22 family. It is involved in the hydrolysis of 1,4-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins. LYZL1 has two isoforms with the molecular weight of 17 kDa and 22 kDa. The full length protein has a signal peptide of 19 amino acids and a glycosylation site. This antibody detects the N-terminal of LYZL1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for LYZL1 antibody 17207-1-AP | Download protocol |
| WB protocol for LYZL1 antibody 17207-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The LYZL1 Polyclonal antibody (Cat# 17207-1-AP) has 1 citation. It was cited in Proteomics Clinical Applications in a study on proteomic analysis of Dpy19l2-deficient human globozoospermia, revealing multiple molecular defects. The research field is reproductive biology and male infertility, with the antibody applied in Western blot for human samples.
| Species | Application | Title |
|---|---|---|
Proteomics Clin Appl Proteomic Analysis of Dpy19l2-Deficient Human Globozoospermia Reveals Multiple Molecular Defects. |
