Laminin beta 1 Monoclonal antibody, PBS Only

• 

  WB analysis using 67705-1-Ig (same clone as 67705-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67705-1-Ig (LAMB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67705-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HeLa using 67705-1-Ig (same clone as 67705-1-PBS)

  WB result of Laminin beta 1 antibody (67705-1-Ig; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-Laminin beta 1 transfected HeLa cells. This data was developed using the same antibody clone with 67705-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 67705-1-Ig (same clone as 67705-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67705-1-Ig (LAMB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67705-1-PBS in a different storage buffer formulation.

• Featured Product
• KD/KO Validated

Laminin beta 1 Monoclonal Antibody for WB, Indirect ELISA

Cat No. 67705-1-PBS

Clone No.1B8E9

Host / Isotype

Mouse / IgG1

Reactivity

human, mouse

Applications

WB, Indirect ELISA

Synonyms

LAMB1, 1B8E9, CLM, Laminin 1 subunit beta, Laminin 10 subunit beta

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Product Information

67705-1-PBS targets Laminin beta 1 in WB, Indirect ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag19182

  Product name: Recombinant human LAMB1 protein

  Source: e coli.-derived, PET28a

  Tag: 6*His

  Domain: 1437-1786 aa of BC113455

  Sequence: AHNAWQKAMDLDQDVLSALAEVEQLSKMVSEAKLRADEAKQSAEDILLKTNATKEKMDKSNEELRNLIKQIRNFLTQDSADLDSIEAVANEVLKMEMPSTPQQLQNLTEDIRERVESLSQVEVILQHSAADIARAEMLLEEAKRASKSATDVKVTADMVKEALEEAEKAQVAAEKAIKQADEDIQGTQNLLTSIESETAASEETLFNASQRISELERNVEELKRKAAQNSGEAEYIEKVVYTVKQSAEDVKKTLDGELDEKYKKVENLIAKKTEESADARRKAEMLQNEAKTLLAQANSKLQLLKDLERKYEDNQRYLEDKAQELARLEGEVRSLLKDISQKVAVYSTCL

• Full Name: laminin, beta 1
• Calculated Molecular Weight: 1786 aa, 198 kDa
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC113455
• Gene Symbol: Laminin beta 1
• Gene ID (NCBI): 3912
• RRID: AB_2882896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• UNIPROT ID: P07942
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

Laminins are a family of basement membrane glycoproteins composed of three nonidentical chains, laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) (PMID: 7959779). Laminins contribute to the structure of extracellular matrix and influence the behavior of associated cells, such as adhesion, differentiation, migration, phenotype stability, and resistance to anoikis (PMID: 23057746). Laminin beta 1 (LAMB1) is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin-1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. Laminin beta 1 is also a subunit of laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213).