Laminin beta 1 Monoclonal antibody

Laminin beta 1 Monoclonal Antibody for WB, ELISA

Host / Isotype

Mouse / IgG1

Reactivity

Human, Mouse

Applications

WB, ELISA

Conjugate

Unconjugated

CloneNo.

1B8E9

Cat no : 67705-1-Ig

Synonyms

CLM, LAMB1, Laminin 1 subunit beta, Laminin 10 subunit beta, Laminin 12 subunit beta, Laminin 2 subunit beta, Laminin 6 subunit beta, Laminin 8 subunit beta, Laminin B1 chain, Laminin beta 1, Laminin subunit beta 1, laminin, beta 1

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Validation Data Gallery

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  WB analysis using 67705-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67705-1-Ig (LAMB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 67705-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67705-1-Ig (LAMB1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Tested Applications

• Positive WB detected in: HEK-293 cells, NIH/3T3 cells, human placenta tissue, U2OS cells, A549 cells, HeLa cells, HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

67705-1-Ig targets Laminin beta 1 in WB, ELISA applications and shows reactivity with Human, Mouse samples.

• Tested Reactivity: Human, Mouse
• Host / Isotype: Mouse / IgG1
• Class: Monoclonal
• Type: Antibody
• Immunogen: Laminin beta 1 fusion protein Ag19182
• Full Name: laminin, beta 1
• Calculated Molecular Weight: 1786 aa, 198 kDa
• Observed Molecular Weight: 220-240 kDa
• GenBank Accession Number: BC113455
• Gene Symbol: LAMB1
• Gene ID (NCBI): 3912
• RRID: AB_2882896
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein G purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Laminins are a family of basement membrane glycoproteins composed of three nonidentical chains, laminin alpha, beta and gamma (formerly A, B1, and B2, respectively) (PMID: 7959779). Laminins contribute to the structure of extracellular matrix and influence the behavior of associated cells, such as adhesion, differentiation, migration, phenotype stability, and resistance to anoikis (PMID: 23057746). Laminin beta 1 (LAMB1) is expressed in most tissues that produce basement membranes, and is one of the 3 chains constituting laminin-1, the first laminin isolated from Engelbreth-Holm-Swarm (EHS) tumor. Laminin beta 1 is also a subunit of laminin-2 (laminin-211 or merosin), laminin-6 (laminin-311 or K-laminin), laminin-8 (laminin-411), laminin-10 (laminin-511) and laminin-12 (laminin-213).