Lipoprotein(a) Polyclonal antibody

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  IHC staining of human placenta using 30675-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 30675-1-AP (Lipoprotein(a) antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 30675-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using Lipoprotein(a) antibody (30675-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

Lipoprotein(a) Polyclonal Antibody for IHC, IF/ICC, ELISA

Cat No. 30675-1-AP

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

IHC, IF/ICC, ELISA

Synonyms

LPA, Lp(a), LP, Apolipoprotein(a), APOA

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Tested Applications

• Positive IHC detected in: human placenta tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

30675-1-AP targets Lipoprotein(a) in IHC, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Lipoprotein(a) fusion protein Ag32907
• Full Name: lipoprotein, Lp(a)
• Calculated Molecular Weight: 501 kDa
• Observed Molecular Weight: 501 kDa
• GenBank Accession Number: NM_005577
• Gene Symbol: LPA
• Gene ID (NCBI): 4018
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P08519
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lipoprotein(a), also known as Lp(a). The plasma concentration of human lipoprotein(a) is correlated with the risk of heart disease. A distinct feature of the Lp(a) particle is the apolipoprotein (a), which is associated with apoB-100, the main protein component of low-density lipoprotein. It is reported that apo(a), which has extensive homology to plasminogen, binds to immobilized fibronectin (PMID: 2531657). The binding of Lp(a) was localized to the C-terminal heparin-binding domain of fibronectin. It is enriched in liver tissue. The N-glycans are complex biantennary structures present in either a mono- or disialylated state.