Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.4 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65296-1-Ig targets Ly-6C in IF, FC applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse |
| Host / Isotype | Rat / IgG2c |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
L3 cloned CTL cells Predict reactive species |
| Full Name | lymphocyte antigen 6 complex, locus C1 |
| GenBank Accession Number | BC010764 |
| Gene Symbol | Ly-6C |
| Gene ID (NCBI) | 17067 |
| RRID | AB_3670287 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P0CW02 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Ly-6C (lymphocyte antigen 6 complex, locus C) is a 14-17 kDa GPI-linked cell surface antigen that belongs to the Ly-6 family of glycosyl phosphatidylinositol-anchored surface glycoproteins (PMID: 9192663). Ly-6C is expressed on mouse monocytes/macrophages, endothelial cells, granulocytes, thymocytes, and some T cell subsets (PMID: 2849552). The two alleles of Ly-6C, Ly-6C.1 and Ly-6C.2, result in differential expression of this protein on subpopulations of CD4+ T cells (PMID: 15057973; 9126982). Ly-6C has been reported as a marker of memory CD8+ T cells (PMID: 7543536).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Ly-6C antibody 65296-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-Mouse Ly-6C (HK1.4), catalog number 65296-1-Ig, has 2 total citations. These appear in Journal of Experimental Medicine and Journal of Leukocyte Biology. The cited research covers liver fibrosis driven by monocyte-derived macrophage expansion in Tet2-deficient mice, and Galectin-3-mediated sialyl Lewis X epitope biosynthesis in monocytes. Both studies used the antibody for immunofluorescence (IF) applications in mouse samples.
| Species | Application | Title |
|---|---|---|
J Exp Med Tet2 deficiency-induced expansion of monocyte-derived macrophages promotes liver fibrosis. | ||
