Tested Applications
| Positive FC detected in | mouse bone marrow cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Flow Cytometry (FC) | FC : 0.5 ug per 10^6 cells in 100 μl suspension |
| This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
65078-1-Ig targets Ly-6G in IHC, IF, FC, Immunohistochemistry applications and shows reactivity with mouse samples.
| Tested Reactivity | mouse |
| Cited Reactivity | mouse, rat |
| Host / Isotype | Rat / IgG2a, kappa |
| Class | Monoclonal |
| Type | Antibody |
| Immunogen |
Ly-6G transfected EL-4J cell line Predict reactive species |
| Full Name | lymphocyte antigen 6 complex, locus G |
| GenBank Accession Number | X70920 |
| Gene Symbol | Ly-6G |
| Gene ID (NCBI) | 546644 |
| RRID | AB_2918382 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Protein G purification |
| UNIPROT ID | P35461 |
| Storage Buffer | PBS with 0.09% sodium azide, pH 7.3. |
| Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for Ly-6G antibody 65078-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The Anti-Mouse Ly-6G (1A8) monoclonal antibody (Cat# 65078-1-Ig) has 46 citations spanning journals including Cell, Nature Communications, Advanced Materials, ACS Nano, Oncogene, Phytomedicine, Journal of Neuroinflammation, Inflammation Research, International Immunopharmacology, Cell Reports, and iScience, among others. Research fields covered include neutrophil biology, NETosis, sepsis, lung and liver injury, ischemic stroke, neuroinflammation, cancer, autoimmune diseases, psoriasis, and periodontitis.
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis | ||
Exploration (Beijing) Targeting eEF1A1 With Baicalein to Block eEF1A1/Prdx4 Interaction for Treating Sepsis-Mediated Lung Injury | ||
Adv Mater Piezoelectric Ceramic Nanofiber Aerogels Direct Neutrophil Fate for Diabetic Tissue Regeneration. | ||
Nat Commun Tudor domain-containing protein 9-targeting siRNA nanoparticles alleviate Pseudomonas aeruginosa lung injury in preclinical models by promoting neutrophil cuproptosis. | ||
ACS Nano Mucoadhesive Nanothalidomide Enema: Simultaneous Radioprotection and Barrier Repair in Radiation Enteritis Therapy. |
Reviews
What customers say
The product received a 5-star rating from a researcher who used it for immunohistochemistry at a 1/400 dilution on mouse kidney tissue. The reviewer described it as a "very good product," indicating strong satisfaction with its performance in their application. Overall, the feedback reflects a positive experience with the antibody's effectiveness and reliability in IHC experiments.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Marion (Verified Customer) (03-11-2025) | Very good product
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