Anti-Mouse Ly-6G (1A8)

Ly-6G Monoclonal Antibody for FC

Host / Isotype

Rat / IgG2a, kappa

Reactivity

Mouse and More (1)

Applications

IHC, IF, FC

Conjugate

Unconjugated

Publications

4

CloneNo.

1A8

Cat no : 65078-1-Ig

Synonyms

Gr1, Gr-1, Ly 6G, Ly6g, Ly-6G

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Validation Data Gallery

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  FC experiment of mouse bone marrow cells using 65078-1-Ig

  1X10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6G (65078-1-Ig, Clone:1A8) and FITC anti-Rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 65078-1-Ig

  1X10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6G (65078-1-Ig, Clone:1A8) and FITC anti-Rat IgG2a Antibody at dilution 1:100, or 0.5 ug Control Antibody. Cells were not fixed.

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below
• IF: See 2 publications below

Product Information

65078-1-Ig targets Ly-6G in IHC, IF, FC applications and shows reactivity with Mouse samples.

• Tested Reactivity: Mouse
• Cited Reactivity: mouse
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen: Ly-6G transfected EL-4J cell line
• Full Name: lymphocyte antigen 6 complex, locus G
• GenBank Accession Number: X70920
• Gene Symbol: Ly-6G/Ly-6C
• Gene ID (NCBI): 546644
• RRID: AB_2918382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• Storage Buffer: PBS with 0.09% sodium azide.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).

Publications

Species	Application	Title
mouse	IF	Eur J Pharmacol; Adelmidrol ameliorates liver ischemia-reperfusion injury through activating Nrf2 signaling pathway; Authors - Min Wu
mouse	IHC	Inflamm Res; Single-cell transcriptome analysis of the mouse lungs during the injury and recovery periods after lipopolysaccharide administration; Authors - Hou-Ping Wang
mouse	IF	Biochim Biophys Acta Mol Basis Dis; JuA alleviates liver ischemia-reperfusion injury by activating AKT/NRF2/HO-1 pathways; Authors - Haoran Fang
mouse	IHC	ACS Omega; Rhoifolin Attenuates Concanavalin A-Induced Autoimmune Hepatitis in Mice via JAKs/STATs Mediated Immune and Apoptotic Processes; Authors - Ge Zhao