Anti-Mouse Ly-6G (1A8)

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  FC experiment of mouse bone marrow cells using 65078-1-Ig

  1X10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6G (65078-1-Ig, Clone:1A8) and FITC anti-Rat IgG2a Antibody at dilution 1:100 (red), or 0.5 ug Control Antibody. Cells were not fixed.

• 

  FC experiment of mouse bone marrow cells using 65078-1-Ig

  1X10^6 mouse bone marrow cells were surface stained with 0.5 ug Anti-Mouse Ly-6G (65078-1-Ig, Clone:1A8) and FITC anti-Rat IgG2a Antibody at dilution 1:100, or 0.5 ug Control Antibody. Cells were not fixed.

Ly-6G Monoclonal Antibody for FC

Cat No. 65078-1-Ig

Clone No.1A8

Publications(15)

Reviews (1)

Host / Isotype

Rat / IgG2a, kappa

Reactivity

mouse and More (1)

Applications

IHC, IF, FC

Synonyms

Ly6g, Ly-6G.1, Ly 6G, 1A8

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Tested Applications

• Positive FC detected in: mouse bone marrow cells

Recommended dilution

• Flow Cytometry (FC): FC : 0.5 ug per 10^6 cells in 100 μl suspension

This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 5 publications below
• IF: See 8 publications below

Product Information

65078-1-Ig targets Ly-6G in IHC, IF, FC applications and shows reactivity with mouse samples.

• Tested Reactivity: mouse
• Cited Reactivity: mouse, rat
• Host / Isotype: Rat / IgG2a, kappa
• Class: Monoclonal
• Type: Antibody
• Immunogen:

  Ly-6G transfected EL-4J cell line

• Full Name: lymphocyte antigen 6 complex, locus G
• GenBank Accession Number: X70920
• Gene Symbol: Ly-6G
• Gene ID (NCBI): 546644
• RRID: AB_2918382
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Affinity purification
• UNIPROT ID: P35461
• Storage Buffer: PBS with 0.09% sodium azide, pH 7.3.
• Storage Conditions: Store at 2-8°C. Stable for one year after shipment.

Background Information

Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	IF	J Adv Res; Astragaloside IV alleviates septic myocardial injury through DUSP1-Prohibitin 2 mediated mitochondrial quality control and ER-autophagy; Authors - Junyan Wang
mouse	IF	Eur J Pharmacol; Adelmidrol ameliorates liver ischemia-reperfusion injury through activating Nrf2 signaling pathway; Authors - Min Wu
mouse	IF	Int Immunopharmacol; Network pharmacology-based strategy to reveal Acacetin against lipopolysaccharide-induced lung injury; Authors - Zhisen Wang
mouse	IF	Signal Transduct Target Ther; Porphyromonas gingivalis aggravates atherosclerotic plaque instability by promoting lipid-laden macrophage necroptosis; Authors - Xiaofei Huang
		Cell Rep Med; A combined "eat me/don't eat me" strategy based on exosome for acute liver injury treatment; Authors - Wei Du
mouse	IHC	Inflamm Res; Single-cell transcriptome analysis of the mouse lungs during the injury and recovery periods after lipopolysaccharide administration; Authors - Hou-Ping Wang

Reviews

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Marion (Verified Customer) (03-11-2025)

Very good product

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1/400
• Cell Tissue Type: Kidney (Mouse)

Ly-6G Antibody Immunohistochemistry validation (1/400 dilution) in Kidney (Mouse) (Cat no:65078-1-Ig)