Anti-Mouse Ly-6G (1A8)
Ly-6G Monoclonal Antibody for FC
Host / Isotype
Rat / IgG2a, kappa
Reactivity
Mouse and More (1)
Applications
IHC, IF, FC
Conjugate
Unconjugated
CloneNo.
1A8
Cat no : 65078-1-Ig
Synonyms
Validation Data Gallery
Tested Applications
Positive FC detected in | mouse bone marrow cells |
Recommended dilution
Application | Dilution |
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This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
Sample-dependent, Check data in validation data gallery. |
Published Applications
IHC | See 1 publications below |
IF | See 2 publications below |
Product Information
65078-1-Ig targets Ly-6G in IHC, IF, FC applications and shows reactivity with Mouse samples.
Tested Reactivity | Mouse |
Cited Reactivity | mouse |
Host / Isotype | Rat / IgG2a, kappa |
Class | Monoclonal |
Type | Antibody |
Immunogen | Ly-6G transfected EL-4J cell line |
Full Name | lymphocyte antigen 6 complex, locus G |
GenBank Accession Number | X70920 |
Gene Symbol | Ly-6G/Ly-6C |
Gene ID (NCBI) | 546644 |
RRID | AB_2918382 |
Conjugate | Unconjugated |
Form | Liquid |
Purification Method | Affinity purification |
Storage Buffer | PBS with 0.09% sodium azide. |
Storage Conditions | Store at 2-8°C. Stable for one year after shipment. |
Background Information
Ly-6G (lymphocyte antigen 6 complex, locus G), also known as Gr-1, is a 21-25 kDa, glycosylphosphatidylinositol-anchored protein expressed on myeloid lineage cells in mouse bone marrow (PMID: 8360469). The expression of Ly-6G increases on neutrophils as they differentiate from immature cells in the bone marrow to mature cells in the blood and spleen (PMID: 8890901). Antibodies targeting Ly6G (RB6-8C5 or 1A8) are commonly used in studies aimed at identifying the role of neutrophils (PMID: 23543767). The 1A8 mAb is specific for Ly-6G (PMID: 8360469).
Protocols
Product Specific Protocols | |
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FC protocol for Ly-6G antibody 65078-1-Ig | Download protocol |
Standard Protocols | |
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Click here to view our Standard Protocols |
Publications
Species | Application | Title |
---|---|---|
Eur J Pharmacol Adelmidrol ameliorates liver ischemia-reperfusion injury through activating Nrf2 signaling pathway | ||
Inflamm Res Single-cell transcriptome analysis of the mouse lungs during the injury and recovery periods after lipopolysaccharide administration | ||
Biochim Biophys Acta Mol Basis Dis JuA alleviates liver ischemia-reperfusion injury by activating AKT/NRF2/HO-1 pathways |