Lysozyme Recombinant monoclonal antibody

• 

  WB analysis of human saliva using 84140-1-RR

  human saliva were subjected to SDS PAGE followed by western blot with 84140-1-RR (Lysozyme antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human tonsillitis using 84140-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84140-1-RR (Lysozyme antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 84140-1-RR

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 84140-1-RR (Lysozyme antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  Affinity and Kinetic Characterization of 84140-1-RR

  Biolayer interferometry (BLl) kinetic assays of 84140-1-RR against Human Lysozyme were performed. The affinity constant is 133 nM.

Lysozyme Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 84140-1-RR

Clone No.241054E1

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, ELISA

Synonyms

LYZ, LZM, Lysozyme C, EC:3.2.1.17, 241054E1

---

Tested Applications

• Positive WB detected in: human saliva
• Positive IHC detected in: human tonsillitis tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

84140-1-RR targets Lysozyme in WB, IHC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Ag6917

  Product name: Recombinant human Lysozyme protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 24-148 aa of BC004147

  Sequence: CELARTLKRLGMDGYRGISLANWMCLAKWESGYNTRATNYNAGDRSTDYGIFQINSRYWCNDGKTPGAVNACHLSCSALLQDNIADAVACAKRVVRDPQGIRAWVAWRNRCQNRDVRQYVQGCGV

• Full Name: lysozyme (renal amyloidosis)
• Calculated Molecular Weight: 17 kDa
• Observed Molecular Weight: 10 kDa
• GenBank Accession Number: BC004147
• Gene Symbol: Lysozyme
• Gene ID (NCBI): 4069
• RRID: AB_3671701
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purfication
• UNIPROT ID: P61626
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

LYZ(Lysozyme C) is also named as LZM and belongs to the glycosyl hydrolase 22 family. It catalyzes the hydrolysis of the bacterial cell wall beta(1-4) glycosidic linkages between N-acetylmuramic acid and N-acetylglucosamine. In tissues and body fluids this protein is associated with the monocyte-macrophage system and enhance the activity of immunoagents. LYZ is capable of both hydrolysis and transglycosylation; it shows also a slight esterase activity. Defects in LYZ are a cause of amyloidosis type 8 (AMYL8). The full length protein has a signal peptide with 18 amino acid.