Tested Applications
| Positive WB detected in | mouse brain tissue |
| Positive IHC detected in | Human Colon cancer tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:200-1:1000 |
| Immunohistochemistry (IHC) | IHC : 1:50-1:500 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
10971-1-AP targets MAB21L2 in WB, IHC, ELISA applications and shows reactivity with human, mouse samples.
| Tested Reactivity | human, mouse |
| Cited Reactivity | human |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag1423 Product name: Recombinant human MAB21L2 protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 1-359 aa of BC009983 Sequence: MIAAQAKLVYQLNKYYTERCQARKAAIAKTIREVCKVVSDVLKEVEVQEPRFISSLSEIDARYEGLEVISPTEFEVVLYLNQMGVFNFVDDGSLPGCAVLKLSDGRKRSMSLWVEFITASGYLSARKIRSRFQTLVAQAVDKCSYRDVVKMIADTSEVKLRIRERYVVQITPAFKCTGIWPRSAAQWPMPHIPWPGPNRVAEVKAEGFNLLSKECYSLTGKQSSAESDAWVLQFGEAENRLLMGGCRNKCLSVLKTLRDRHLELPGQPLNNYHMKTLLLYECEKHPRETDWDESCLGDRLNGILLQLISCLQCRRCPHYFLPNLDLFQGKPHSALESAAKQTWRLAREILTNPKSLDKL Predict reactive species |
| Full Name | mab-21-like 2 (C. elegans) |
| Calculated Molecular Weight | 41 kDa |
| Observed Molecular Weight | 41 kDa |
| GenBank Accession Number | BC009983 |
| Gene Symbol | MAB21L2 |
| Gene ID (NCBI) | 10586 |
| RRID | AB_2139234 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y586 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
Mab-21 gene has been identified as a critical component required for sensory organ identity establishment in Caenorhabditis elegans. Mab21l1 and Mab21l2 paralogs have widespread and dynamic expression patterns during vertebrate development. Depletion of Mab21l1 and Mab21l2 messages in mouse embryo arrests axial turning, and impairs notochord and neural tube differentiation. Human MAB21L2 gene encodes mab-21-like 2 protein belonging to the mab-21 family. The Mab21l2-deficient mice have defects in eye and body wall formation, indicating that Mab21l2 plays crucial roles in retina and in ventral body wall formation. MAB21L2 plays a crucial role in both heart and liver development during mouse embryogenesis. This antibody is supposed to recognize both MAB21L2 and its homolog MAB21L1.
Protocols
| Product Specific Protocols | |
|---|---|
| IHC protocol for MAB21L2 antibody 10971-1-AP | Download protocol |
| WB protocol for MAB21L2 antibody 10971-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
The MAB21L2 Polyclonal antibody (catalog number 10971-1-AP) has 1 citation. It appears in the journal Cancers (Basel), in a study on STAMP2 expression mediated by cytokines in prostate cancer cells. The research field is cancer biology, specifically prostate cancer, with the antibody used in Western blot (WB) on human samples.
| Species | Application | Title |
|---|---|---|
Cancers (Basel) STAMP2 Expression Mediated by Cytokines Attenuates Their Growth-Limiting Effects in Prostate Cancer Cells. |
Reviews
What customers say
The single review for this antibody reports a negative Western Blot result. The user tested dilutions of 1:200 and 1:100 on E12.5 mouse head tissue expressing an epitope-tagged target, but no band appeared at the expected molecular weight. Instead, a non-specific band was observed at approximately 66 kDa, likely corresponding to BSA. The reviewer concluded that the antibody failed to detect the protein of interest in their experimental setup.
The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.
Kathryn (Verified Customer) (04-07-2023) | No band appeared where expected. The antibody produced a band in the protein ladder at approximately 66 kDa, possibly BSA. Ultimately, this antibody does not detect the protein of interest
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