MAdCAM1 Monoclonal antibody

MAdCAM1 Monoclonal Antibody for IF, IHC, ELISA

Host / Isotype

Mouse / IgG2a

Reactivity

Human and More (1)

Applications

IHC, IF, ELISA

Conjugate

Unconjugated

Publications

2

CloneNo.

2A12E8

Cat no : 66594-1-Ig

Synonyms

hMAdCAM 1, MACAM1, MAdCAM 1, MAdCAM1

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Validation Data Gallery

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  IHC staining of human colon using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human small intestine using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 10x lens).

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  IHC staining of human small intestine using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human small intestine tissue slide using 66594-1-Ig (MADCAM1 antibody) at dilution of 1:500 (under 40x lens).

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  IHC staining of human appendicitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with .

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  IHC staining of human appendicitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human appendicitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with .

• 

  IHC staining of human tonsillitis using 66594-1-Ig

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 66594-1-Ig (MAdCAM1 antibody) at dilution of 1:500 (under 4x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of human tonsillitis using 66594-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

• 

  IF Staining of human tonsillitis using 66594-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using MAdCAM1 antibody (66594-1-Ig, Clone: 2A12E8 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Tested Applications

• Positive IHC detected in: human colon tissue, human tonsillitis tissue, human appendicitis tissue, human small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunohistochemistry (IHC): IHC : 1:1000-1:4000
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 2 publications below

Product Information

66594-1-Ig targets MAdCAM1 in IHC, IF, ELISA applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Cited Reactivity: human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAdCAM1 fusion protein Ag16184
• Full Name: mucosal vascular addressin cell adhesion molecule 1
• Calculated Molecular Weight: 406 aa, 43 kDa
• GenBank Accession Number: BC142629
• Gene Symbol: MADCAM1
• Gene ID (NCBI): 8174
• RRID: AB_2881954
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.

Publications

Species	Application	Title
human	IHC	J Crohns Colitis; Mucosal addressin cell adhesion molecule 1 expression reflects mucosal inflammation and subsequent relapse in patients with ulcerative colitis; Authors - Kazuhiko Uchiyama
human	IHC	Int J Cancer; MAdCAM-1 targeting strategy can prevent colitic cancer carcinogenesis and progression via suppression of immune cell infiltration and inflammatory signals; Authors - Naoya Ozawa