CoraLite®594-conjugated MAdCAM1 Monoclonal antibody

MAdCAM1 Monoclonal Antibody for IF

Host / Isotype

Mouse / IgG2a

Reactivity

Human

Applications

IF

Conjugate

CoraLite®594 Fluorescent Dye

CloneNo.

2A12E8

Cat no : CL594-66594

Synonyms

hMAdCAM 1, MACAM1, MAdCAM 1, MADCAM1

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Validation Data Gallery

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  IF Staining of human tonsillitis using CL594-66594

  Immunofluorescent analysis of (4% PFA) fixed human tonsillitis tissue using CoraLite®594 MAdCAM1 antibody (CL594-66594, Clone: 2A12E8 ) at dilution of 1:200.

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Tested Applications

• Positive IF detected in: human tonsillitis tissue

Recommended dilution

• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL594-66594 targets MAdCAM1 in IF applications and shows reactivity with Human samples.

• Tested Reactivity: Human
• Host / Isotype: Mouse / IgG2a
• Class: Monoclonal
• Type: Antibody
• Immunogen: MAdCAM1 fusion protein Ag16184
• Full Name: mucosal vascular addressin cell adhesion molecule 1
• Calculated Molecular Weight: 406 aa, 43 kDa
• GenBank Accession Number: BC142629
• Gene Symbol: MADCAM1
• Gene ID (NCBI): 8174
• RRID: AB_2920014
• Conjugate: CoraLite®594 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 588 nm / 604 nm
• Form: Liquid
• Purification Method: Protein A purification
• Storage Buffer: PBS with 50% Glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

Lymphocyte recirculation through different organs is thought to be regulated by adhesion molecules ("homing receptors") recognizing tissue-specific vascular addressins on endothelium (PMID: 7511642). The mucosal vascular addressin, MAdCAM1, is an immunoglobulin superfamily adhesion receptor for lymphocytes that is expressed by mucosal venules and helps direct lymphocyte traffic into mucosal tissues including the Peyer patches and the intestinal lamina propria (PMID: 7687523). MAdCAM1 can bind both integrin alpha-4/beta-7 and L-selectin, regulating both the passage and retention of leukocytes.