Tested Applications
| Positive WB detected in | RAW 264.7 cells |
| Positive IHC detected in | mouse pancreas tissue Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 |
| Positive IF/ICC detected in | U-251 cells |
| Positive FC (Intra) detected in | RAW 264.7 cells |
Recommended dilution
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:500-1:2000 |
| Immunohistochemistry (IHC) | IHC : 1:250-1:1000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, Check data in validation data gallery. | |
Product Information
20189-1-AP targets MAFB in WB, IHC, IF/ICC, FC (Intra), IP, ChIP, ELISA applications and shows reactivity with human, mouse, rat samples.
| Tested Reactivity | human, mouse, rat |
| Cited Reactivity | human, mouse, rat |
| Host / Isotype | Rabbit / IgG |
| Class | Polyclonal |
| Type | Antibody |
| Immunogen |
CatNo: Ag14102 Product name: Recombinant human MAFB protein Source: e coli.-derived, PGEX-4T Tag: GST Domain: 168-323 aa of BC036689 Sequence: QASPPPSSAASPAQQLPTSHPGPGPHATASATAAGGNGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL Predict reactive species |
| Full Name | v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian) |
| Calculated Molecular Weight | 323 aa, 36 kDa |
| Observed Molecular Weight | 45 kDa |
| GenBank Accession Number | BC036689 |
| Gene Symbol | MAFB |
| Gene ID (NCBI) | 9935 |
| ENSEMBL Gene ID | ENSG00000204103 |
| RRID | AB_2878654 |
| Conjugate | Unconjugated |
| Form | Liquid |
| Purification Method | Antigen affinity purification |
| UNIPROT ID | Q9Y5Q3 |
| Storage Buffer | PBS with 0.02% sodium azide and 50% glycerol, pH 7.3. |
| Storage Conditions | Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA. |
Background Information
MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).
Protocols
| Product Specific Protocols | |
|---|---|
| FC protocol for MAFB antibody 20189-1-AP | Download protocol |
| IF protocol for MAFB antibody 20189-1-AP | Download protocol |
| IHC protocol for MAFB antibody 20189-1-AP | Download protocol |
| WB protocol for MAFB antibody 20189-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
What published studies show
Anti-MAFB (Cat# 20189-1-AP) has 10 total citations spanning journals including J Exp Clin Cancer Res, Communications Biology, Frontiers in Microbiology, Journal of Leukocyte Biology, Experimental Cell Research, Molecular and Cellular Endocrinology, BMC Cancer, Journal of Orthopaedic Translation, and Research. Research fields cover pancreatic cancer EMT, pulmonary fibrosis, spinal cord injury, NLRP3 inflammasome activation, tuberculosis immunity, bone resorption, adipose browning, hepatic steatosis, amyloid-β phagocytosis, and glioblastoma immunology.
| Species | Application | Title |
|---|---|---|
J Exp Clin Cancer Res Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling | ||
Research (Wash D C) Monocyte-Derived LGMN(+) Macrophages Divert Lung Injury Outcomes toward Fibrosis through Matrix Remodeling. | ||
J Orthop Translat Profibrotic macrophage-derived CXCL4 promotes pericyte-to-myofibroblast transition after spinal cord injury. | ||
Commun Biol The matrix vesicle cargo miR-125b accumulates in the bone matrix, inhibiting bone resorption in mice | ||
Front Microbiol Transcription factor MAFB controls type I and II interferon response-mediated host immunity in Mycobacterium tuberculosis-infected macrophages
| ||
Commun Biol MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages |
