MAFB Polyclonal antibody

• 

  WB analysis of RAW 264.7 using 20189-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 20189-1-AP (MAFB Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of U-251 using 20189-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using MAFB antibody (20189-1-AP) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

• 

  FC experiment of RAW 264.7 using 20189-1-AP

  1X10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Anti-Human MAFB (20189-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

MAFB Polyclonal Antibody for WB, IF/ICC, FC (Intra), ELISA

Cat No. 20189-1-AP

Publications(6)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IF/ICC, FC (Intra), IP, ELISA

Synonyms

KRML, Maf B, Maf-B, Transcription factor MafB, V-maf musculoaponeurotic fibrosarcoma oncogene homolog B

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Tested Applications

• Positive WB detected in: RAW 264.7 cells
• Positive IF/ICC detected in: U-251 cells
• Positive FC (Intra) detected in: RAW 264.7 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• KD/KO: See 2 publications below
• WB: See 6 publications below
• IF: See 1 publications below
• IP: See 1 publications below

Product Information

20189-1-AP targets MAFB in WB, IF/ICC, FC (Intra), IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14102

  Product name: Recombinant human MAFB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 168-323 aa of BC036689

  Sequence: QASPPPSSAASPAQQLPTSHPGPGPHATASATAAGGNGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL

• Full Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC036689
• Gene Symbol: MAFB
• Gene ID (NCBI): 9935
• ENSEMBL Gene ID: ENSG00000204103
• RRID: AB_2878654
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5Q3
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IF	J Leukoc Biol; Glia maturation factor-γ regulates amyloid-β42 phagocytosis through scavenger receptor AI in murine macrophages; Authors - Wulin Aerbajinai
human	WB	Front Microbiol; Transcription factor MAFB controls type I and II interferon response-mediated host immunity in Mycobacterium tuberculosis-infected macrophages; Authors - Haruka Hikichi ; KD Validated
human	WB	J Exp Clin Cancer Res; Musashi2 promotes EGF-induced EMT in pancreatic cancer via ZEB1-ERK/MAPK signaling; Authors - Weiwei Sheng
rat	WB	Mol Cell Endocrinol; lnc-HC ameliorates steatosis by promoting miR-130b-3p biogenesis and the assembly of an RNA-induced silencing complex; Authors - Xi Lan ; KD Validated
mouse	WB,IP	Commun Biol; MafB regulates NLRP3 inflammasome activation by sustaining p62 expression in macrophages; Authors - Huachun Cui
mouse	WB	Commun Biol; The matrix vesicle cargo miR-125b accumulates in the bone matrix, inhibiting bone resorption in mice; Authors - Tomoko Minamizaki