CoraLite® Plus 488-conjugated MAFB Polyclonal antibody

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  FC experiment of RAW 264.7 using CL488-20189

  1x10^6 RAW 264.7 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 MAFB Polyclonal Antibody (CL488-20189)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

• Featured Product
• KD/KO Validated

MAFB Polyclonal Antibody for FC (Intra)

Cat No. CL488-20189

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

FC (Intra)

Synonyms

V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, Transcription factor MafB, Maf-B, Maf B, KRML

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Tested Applications

• Positive FC (Intra) detected in: RAW 264.7 cells

Recommended dilution

• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

CL488-20189 targets MAFB in FC (Intra) applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag14102

  Product name: Recombinant human MAFB protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 168-323 aa of BC036689

  Sequence: QASPPPSSAASPAQQLPTSHPGPGPHATASATAAGGNGSVEDRFSDDQLVSMSVRELNRHLRGFTKDEVIRLKQKRRTLKNRGYAQSCRYKRVQQKHHLENEKTQLIQQVEQLKQEVSRLARERDAYKVKCEKLANSGFREAGSTSDSPSSPEFFL

• Full Name: v-maf musculoaponeurotic fibrosarcoma oncogene homolog B (avian)
• Calculated Molecular Weight: 323 aa, 36 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC036689
• Gene Symbol: MAFB
• Gene ID (NCBI): 9935
• ENSEMBL Gene ID: ENSG00000204103
• RRID: AB_3672714
• Conjugate: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission Maxima Wavelengths: 493 nm / 522 nm
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9Y5Q3
• Storage Buffer: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA, pH 7.3.
• Storage Conditions: Store at -20°C. Avoid exposure to light. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage.

Background Information

MAFB (MAF BZIP Transcription Factor B), also known as V-maf musculoaponeurotic fibrosarcoma oncogene homolog B, is a widely expressed basic leucine zipper transcription factor belonging to the Maf family. MAFB expression is significantly elevated in response to a broad range of metabolic and immunological stimuli that promote macrophage M2 polarization, cholesterol efflux, or both, whereas it is strongly down-regulated in response to various pro-inflammatory pathogenic triggers(PMID: 28790455). MAFB is highly expressed in tumor tissues and required for proliferation and tumorigenicity of osteosarcoma cells(PMID: 32234710) and is a marker of tumor-associated macrophages in both mouse and human(PMID: 31679694).