MAG/Siglec-4a Recombinant monoclonal antibody

• 

  WB analysis using 86073-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86073-2-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86073-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86073-2-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 86073-2-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86073-2-RR (MAG/Siglec-4a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of rat brain using 86073-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86073-2-RR (MAG/Siglec-4a antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 86073-2-RR

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 86073-2-RR (MAG/Siglec-4a antibody) at dilution of 1:1000 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

MAG/Siglec-4a Uni-rAb^® Recombinant Antibody for WB, IHC, ELISA

Cat No. 86073-2-RR

Clone No.250740G9

Host / Isotype

Rabbit / IgG

Reactivity

mouse, rat

Applications

WB, IHC, ELISA

Synonyms

1B236, Brain neuron cytoplasmic protein 3, Mag, Myelin-associated glycoprotein, Sialic acid-binding Ig-like lectin 4a

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Tested Applications

• Positive WB detected in: rat brain tissue, unboiled rat brain tissue, rat cerebellum tissue, mouse brain tissue, rat spinal cord tissue
• Positive IHC detected in: rat brain tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochemistry (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86073-2-RR targets MAG/Siglec-4a in WB, IHC, ELISA applications and shows reactivity with mouse, rat samples.

• Tested Reactivity: mouse, rat
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  CatNo: Eg3251

  Product name: Recombinant Rat MAG protein (rFc Tag)

  Source: mammalian cells-derived, pHZ-KIsec-C-rFc

  Tag: C-rFc

  Domain: 20-516 aa of NM_017190.4

  Sequence: GHWGAWMPSSISAFEGTCVSIPCRFDFPDELRPAVVHGVWYFNSPYPKNYPPVVFKSRTQVVHESFQGRSRLLGDLGLRNCTLLLSTLSPELGGKYYFRGDLGGYNQYTFSEHSVLDIINTPNIVVPPEVVAGTEVEVSCMVPDNCPELRPELSWLGHEGLGEPTVLGRLREDEGTWVQVSLLHFVPTREANGHRLGCQAAFPNTTLQFEGYASLDVKYPPVIVEMNSSVEAIEGSHVSLLCGADSNPPPLLTWMRDGMVLREAVAESLYLDLEEVTPAEDGIYACLAENAYGQDNRTVELSVMYAPWKPTVNGTVVAVEGETVSILCSTQSNPDPILTIFKEKQILATVIYESQLQLELPAVTPEDDGEYWCVAENQYGQRATAFNLSVEFAPIILLESHCAAARDTVQCLCVVKSNPEPSVAFELPSRNVTVNETEREFVYSERSGLLLTSILTLRGQAQAPPRVICTSRNLYGTQSLELPFQGAHRLMWAKIGP

• Full Name: myelin-associated glycoprotein
• Calculated Molecular Weight: 69 kDa
• Observed Molecular Weight: 70-100 kDa
• GenBank Accession Number: NM_017190.4
• Gene Symbol: Mag
• Gene ID (NCBI): 29409
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P07722-1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.