MAGEA1 Polyclonal antibody

MAGEA1 Polyclonal Antibody for IF, WB,ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF,ELISA

Conjugate

Unconjugated

Cat no : 55105-1-AP

Synonyms

Antigen MZ2 E, Cancer/testis antigen 1.1, CT1.1, MAGE 1 antigen, MAGE1, MAGE1A, MAGEA1, Melanoma associated antigen 1

---

Validation Data Gallery

• 

  WB analysis of A375 using 55105-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 55105-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 55105-1-AP (MAGEA1 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  IF Staining of A375 using 55105-1-AP

  Immunofluorescent analysis of A375 cells using 55105-1-AP (MAGEA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

---

Tested Applications

• Positive WB detected in: A375 cells, MCF-7 cells
• Positive IF detected in: A375 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

55105-1-AP targets MAGEA1 in WB, IF,ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: Peptide
• Full Name: melanoma antigen family A, 1 (directs expression of antigen MZ2-E)
• Calculated Molecular Weight: 34 kDa
• Observed Molecular Weight: 46 kDa
• GenBank Accession Number: NM_004988
• Gene Symbol: MAGEA1
• Gene ID (NCBI): 4100
• RRID: AB_10859942
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGEA1, also named as CT1.1, MZ2-E, MAGE1 and MAGE1A, is the first gene reported to encode a CTL-defined human tumor antigen. It may play a role in embryonal development and tumor transformation or aspects of tumor progression. Antigen recognized on a melanoma by autologous cytolytic T-lymphocytes. MAGEA1 provided direct evidence for the power of the cDNA expression library as an effective strategy to identify genes encoding tumor antigens recognized by HLA-restricted T cells.(PMID:16951217 ) The antibody is specific to MAGEA1.