MAGEA4 Polyclonal antibody

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  WB analysis using 12508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of human lung cancer using 12508-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12508-1-AP (MAGEA4 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 12508-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 12508-1-AP (MAGEA4 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 12508-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12508-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).

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  FC experiment of A431 using 12508-1-AP

  1x10^6 A431 cells were intracellularly stained with 0.4 ug MAGEA4 Polyclonal antibody (12508-1-AP) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

MAGEA4 Polyclonal Antibody for WB, IHC, FC (Intra), ELISA

Cat No. 12508-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC (Intra), ELISA

Synonyms

MAGE X2, MAGE 41 antigen, MAGE 41, MAGE 4 antigen, CT1.4

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Tested Applications

• Positive WB detected in: A431 cells, U2OS cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC (Intra) detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• IHC: See 1 publications below

Product Information

12508-1-AP targets MAGEA4 in WB, IHC, FC (Intra), ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Cited Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEA4 fusion protein Ag3193
• Full Name: melanoma antigen family A, 4
• Calculated Molecular Weight: 317 aa, 35 kDa
• Observed Molecular Weight: 45 kDa
• GenBank Accession Number: BC017723
• Gene Symbol: MAGEA4
• Gene ID (NCBI): 4103
• RRID: AB_2877863
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: P43358
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAGEA4, also named MAGE4 and CT1.4, is one of the germ cell-specific markers (PMID:16540528). SMAGEA4 is one of the subgroup I MAGEAs members which may play important roles in the immune surveillance of certain tumor types and play important roles during embryogenesis.(PMID:15222021) The antibody is specific to MAGEA4.

Publications

Species	Application	Title
human	IHC	Front Endocrinol (Lausanne); Four novel mutations identification in 17 beta-hydroxysteroid dehydrogenase-3 deficiency and our clinical experience: possible benefits of early treatment; Authors - Yunpeng Wang