MAGEA4 Polyclonal antibody

MAGEA4 Polyclonal Antibody for FC, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, FC, ELISA

Conjugate

Unconjugated

Cat no : 12508-1-AP

Synonyms

Cancer/testis antigen 1.4, CT1.4, MAGE 4 antigen, MAGE 41, MAGE 41 antigen, MAGE X2, MAGE X2 antigen, MAGE4, MAGE4A, MAGE4B, MAGEA4, melanoma antigen family A, 4, Melanoma associated antigen 4

---

Validation Data Gallery

• 

  WB analysis using 12508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12508-1-AP (MAGEA4 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human breast cancer using 12508-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 12508-1-AP (MAGEA4 antibody) at dilution of 1:50 (under 10x lens).

• 

  FC experiment of A431 using 12508-1-AP

  1X10^6 A431 cells were stained with 0.2 ug Anti-Human MAGEA4 (12508-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (green), or stained with 0.2 ug isotype control and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (black). Cells were fixed with 90% MeOH.

---

Tested Applications

• Positive WB detected in: A431 cells, U2OS cells
• Positive IHC detected in: human breast cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive FC detected in: A431 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochemistry (IHC): IHC : 1:20-1:200

Sample-dependent, check data in validation data gallery

Product Information

12508-1-AP targets MAGEA4 in WB, IHC, FC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen: MAGEA4 fusion protein Ag3193
• Full Name: melanoma antigen family A, 4
• Calculated molecular weight: 317 aa, 35 kDa
• Observed molecular weight: 45 kDa
• GenBank accession number: BC017723
• Gene symbol: MAGEA4
• Gene ID (NCBI): 4103
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.