MAGEA6 Polyclonal antibody

MAGEA6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14602-1-AP

Synonyms

Cancer/testis antigen 1.6, CT1.6, MAGE 3b, MAGE 6 antigen, MAGE3B, MAGE3B antigen, MAGE6, MAGEA6, melanoma antigen family A, 6, Melanoma associated antigen 6



Tested Applications

Positive WB detected inmouse testis tissue, A375 cells, rat testis tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:3000
Immunohistochemistry (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)IF : 1:10-1:100
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, Check data in validation data gallery.

Product Information

14602-1-AP targets MAGEA6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse, rat samples.

Tested Reactivity human, mouse, rat
Cited Reactivityhuman
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA6 fusion protein Ag6148
Full Name melanoma antigen family A, 6
Calculated Molecular Weight 35 kDa
Observed Molecular Weight45-50 kDa
GenBank Accession NumberBC067731
Gene Symbol MAGEA6
Gene ID (NCBI) 4105
RRIDAB_2250093
Conjugate Unconjugated
Form Liquid
Purification MethodAntigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage. 20ul sizes contain 0.1% BSA.

Protocols

Product Specific Protocols
WB protocol for MAGEA6 antibody 14602-1-APDownload protocol
IHC protocol for MAGEA6 antibody 14602-1-APDownload protocol
IF protocol for MAGEA6 antibody 14602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Exp Neurol

Helios modulates the maturation of a CA1 neuronal subpopulation required for spatial memory formation.

Authors - Albert Giralt
humanIHC

Anticancer Res

Tissue Expression of Melanoma-associated Antigen A6 and Clinical Characteristics of Gastric Cancer.

Authors - Miyo Endo
humanWB

Exp Ther Med

MAGEA6 positively regulates MSMO1 and promotes the migration and invasion of oesophageal cancer cells.

Authors - Manyu Liu
  • KD Validated
humanIHC

Exp Ther Med

Investigation into the expression levels of MAGEA6 in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissues.

Authors - Jun Hao