MAGEA6 Polyclonal antibody

MAGEA6 Polyclonal Antibody for IF, IHC, WB, ELISA

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, IHC, IF, ELISA

Conjugate

Unconjugated

Cat no : 14602-1-AP

Synonyms

Cancer/testis antigen 1.6, CT1.6, MAGE 3b, MAGE 6 antigen, MAGE3B, MAGE3B antigen, MAGE6, MAGEA6, melanoma antigen family A, 6, Melanoma associated antigen 6



Tested Applications

Positive WB detected inHepG2 cells, A375 cells, HeLa cells, MCF-7 cells, mouse testis tissue
Positive IHC detected inhuman lung cancer tissue, human breast cancer tissue
Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
Positive IF detected inA375 cells

Recommended dilution

ApplicationDilution
Western Blot (WB)WB : 1:500-1:1000
Immunohistochemistry (IHC)IHC : 1:20-1:200
Immunofluorescence (IF)IF : 1:10-1:100
Sample-dependent, check data in validation data gallery

Published Applications

IHCSee 3 publications below

Product Information

14602-1-AP targets MAGEA6 in WB, IHC, IF, ELISA applications and shows reactivity with human, mouse samples.

Tested Reactivity human, mouse
Cited Reactivity human
Host / Isotype Rabbit / IgG
Class Polyclonal
Type Antibody
Immunogen MAGEA6 fusion protein Ag6148
Full Name melanoma antigen family A, 6
Calculated molecular weight 35 kDa
Observed molecular weight 66-70 kDa
GenBank accession numberBC067731
Gene symbol MAGEA6
Gene ID (NCBI) 4105
Conjugate Unconjugated
Form Liquid
Purification Method Antigen affinity purification
Storage Buffer PBS with 0.02% sodium azide and 50% glycerol pH 7.3.
Storage ConditionsStore at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20oC storage.

Protocols

Product Specific Protocols
WB protocol for MAGEA6 antibody 14602-1-APDownload protocol
IHC protocol for MAGEA6 antibody 14602-1-APDownload protocol
IF protocol for MAGEA6 antibody 14602-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
humanIHC

Exp Ther Med

Investigation into the expression levels of MAGEA6 in esophageal squamous cell carcinoma and esophageal adenocarcinoma tissues.

Authors - Jun Hao
humanIHC

Anticancer Res

Tissue Expression of Melanoma-associated Antigen A6 and Clinical Characteristics of Gastric Cancer.

Authors - Miyo Endo
humanIHC

Exp Neurol

Helios modulates the maturation of a CA1 neuronal subpopulation required for spatial memory formation.

Authors - Albert Giralt