MAGED2 Polyclonal antibody

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• MAGED2 Polyclonal antibody (15252-1-AP)

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• KD/KO Validated

MAGED2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Cat No. 15252-1-AP Copied!

Publications (6)

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Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, IP, ELISA

Synonyms

11B6, BCG-1, Breast cancer-associated gene 1 protein, HCA10, Hepatocellular carcinoma-associated protein JCL-1

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  WB analysis using 15252-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  WB analysis of HepG2 using 15252-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of A431 using 15252-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  WB analysis of MCF-7 using 15252-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 15252-1-AP (MAGED2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IP experiment of HepG2 using 15252-1-AP

  IP result of anti-MAGED2 (IP:15252-1-AP, 4ug; Detection:15252-1-AP 1:1000) with HepG2 cells lysate 3200ug.

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
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  IHC staining of human breast cancer using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human breast cancer using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of human placenta using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse kidney using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse pancreas using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of mouse small intestine using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat kidney using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat pancreas using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded rat pancreas tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IHC staining of rat small intestine using 15252-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 15252-1-AP (MAGED2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy
• 

  IF Staining of MCF-7 using 15252-1-AP

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using MAGED2 antibody (15252-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), F-actin was stained using CoraLite®594-phalloidin (red) and DNA was stained by DAPI (blue).

  This validation data has been generated in-house and reviewed by Proteintech scientists. Find out more on our antibody validation policy

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Tested Applications

• Positive WB detected in: HEK-293 cells, HepG2 cells, A431 cells, MCF-7 cells, mouse liver tissue, rat liver tissue
• Positive IP detected in: HepG2 cells
• Positive IHC detected in: human breast cancer tissue, mouse small intestine tissue, mouse pancreas tissue, rat kidney tissue, mouse kidney tissue, rat pancreas tissue, human placenta tissue, rat small intestine tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: MCF-7 cells

Recommended dilution

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprecipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochemistry (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

15252-1-AP targets MAGED2 in WB, IHC, IF/ICC, IP, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag6828

  Product name: Recombinant human MAGED2 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 151-479 aa of BC000304

  Sequence: DEPEPESAAAQSQENQDTRPKVKAKKARKVKHLDGEEDGSSDQSQASGTTGGRRVSKALMASMARRASRGPIAFWARRASRTRLAAWARRALLSLRSPKARRGKARRRAAKLQSSQEPEAPPPRDVALLQGRANDLVKYLLAKDQTKIPIKRSDMLKDIIKEYTDVYPEIIERAGYSLEKVFGIQLKEIDKNDHLYILLSTLEPTDAGILGTTKDSPKLGLLMVLLSIIFMNGNRSSEAVIWEVLRKLGLRPGIHHSLFGDVKKLITDEFVKQKYLDYARVPNSNPPEYEFFWGLRSYYETSKMKVLKFACKVQKKDPKEWAAQYREAM

• Full Name: melanoma antigen family D, 2
• Calculated Molecular Weight: 65 kDa
• Observed Molecular Weight: 65 kDa
• GenBank Accession Number: BC000304
• Gene Symbol: MAGED2
• Gene ID (NCBI): 10916
• RRID: AB_2138557
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9UNF1
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

What published studies show

Anti-MAGED2 antibody (Cat# 15252-1-AP) has 6 total citations published in Nephrol Dial Transplant, Biochem Pharmacol, mBio, J Neurosci, RNA, and Biochim Biophys Acta. Research fields span kidney injury, cell cycle and DNA damage response, SARS-CoV-2 antiviral defense, serotonin-mediated depression behavior, RNA guanine quadruplex/TRAIL sensitization, and nucleolar protein dynamics under cellular stress. Applications include WB, IHC, IP, and IF in human and mouse samples.

Automatically generated from the 6 publication citations by Able AI.

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
mouse	WB,IHC	Nephrol Dial Transplant; MAGE genes in the kidney: identification of MAGED2 as upregulated during kidney injury and in stressed tubular cells.; Authors - Lara Valiño-Rivas
human	WB	Biochem Pharmacol; Melanoma antigen-D2 controls cell cycle progression and modulates the DNA damage response.; Authors - Charlotte Trussart ; KD Validated
human	WB,IP	mBio; SARS-CoV-2 main protease cleaves MAGED2 to antagonize host antiviral defense; Authors - Xiaohui Ju ; KD Validated
human	WB	RNA; An RNA guanine quadruplex regulated pathway to TRAIL-sensitization by DDX21.; Authors - Ewan K S McRae
mouse	WB	J Neurosci; MAGE-D1 regulates expression of depression-like behavior through serotonin transporter ubiquitylation.; Authors - Mouri Akihiro A
human	WB,IF	Biochim Biophys Acta; Melanoma antigen-D2: A nucleolar protein undergoing delocalization during cell cycle and after cellular stress.; Authors - Céline Pirlot ; KD Validated