MAGED4 Polyclonal antibody, PBS Only

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• MAGED4 Polyclonal antibody, PBS Only (16809-1-PBS)

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  WB analysis of Jurkat using 16809-1-AP (same clone as 16809-1-PBS)

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 16809-1-AP (same clone as 16809-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 16809-1-AP (MAGED4 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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  IHC staining of human gliomas using 16809-1-AP (same clone as 16809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 16809-1-AP (MAGED4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

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  IHC staining of human heart using 16809-1-AP (same clone as 16809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

• 

  IHC staining of human heart using 16809-1-AP (same clone as 16809-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16809-1-AP (MAGED4 Antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 16809-1-PBS in a different storage buffer formulation.

MAGED4 Polyclonal Antibody for WB, IHC, Indirect ELISA

Cat No. 16809-1-PBS

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IHC, Indirect ELISA

Synonyms

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Product Information

16809-1-PBS targets MAGED4 in WB, IHC, Indirect ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag10329

  Product name: Recombinant human MAGED4 protein

  Source: e coli.-derived, T-HIS

  Tag: 6*His

  Domain: 1-350 aa of BC081566

  Sequence: MAEGSFSVQSESYSVEDMDEGSDEVGEEEMVEGNDYEEFGAFGGYGTLTSFDIHILRAFGSLGPGLRILSNEPWELENPVLAQTLVEALQLDPETLANETAARAANVARAAASNRAARAAAAAARTAFSQVVASHRVATPQVSGEDTQPTTYAAEAQGPTPEPPLASPQTSQMLVTSKMAAPEAPATSAQSQTGSPAQEAATEGPSSACAFSQAPCAREVDANRPSTAFLGQNDVFDFTQPAGVSGMAFPRPKRPAPAQEAATEGPSAASGVPQTGPGREVAATRPKTTKSGKALAKTRWVEPQNVVAAAAAKAKMATSIPEPEGAAAATAQHSAEPWARMGGKRTKKSK

• Full Name: melanoma antigen family D, 4
• Calculated Molecular Weight: 741 aa, 81 kDa
• Observed Molecular Weight: 90-100 kDa
• GenBank Accession Number: BC081566
• Gene Symbol: MAGED4
• Gene ID (NCBI): 728239
• RRID: AB_2138678
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q96JG8
• Storage Buffer: PBS only, pH 7.3.
• Storage Conditions: Store at -80°C.

Background Information

MAGED4, originally termed MAGEE1, is a member of MAGE (Melanoma associated antigen) super-family identified by serial analysis of gene expression (SAGE) technique (PMID: 11406556; 24966945). Three isoforms (MAGED4a, b, and c) were produced by alternative splice in the process of gene transcription (PMID: 11406556). This antibody raised against 1-350 aa of human MAGED4 protein detects a band of 90-100 kDa, which is larger than the calculated molecular weight. The slower electrophoretic mobility may be due to abnormal spatial conformation of MAGED4 protein caused by abundance of proline in composition (PMID: 24966945).