MAGEH1 Polyclonal antibody

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  WB analysis of Y79 using 12424-1-AP

  Y79 cells were subjected to SDS PAGE followed by western blot with 12424-1-AP (MAGEH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 12424-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12424-1-AP (MAGEH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-3 using 12424-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 12424-1-AP (MAGEH1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human prostate cancer using 12424-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12424-1-AP (MAGEH1 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 12424-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 12424-1-AP (MAGEH1 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human prostate cancer using 12424-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 12424-1-AP (MAGEH1 antibody) at dilution of 1:50 (under 10x lens).

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  IF Staining of PC-3 using 12424-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed PC-3 cells using 12424-1-AP (MAGEH1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

MAGEH1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Cat No. 12424-1-AP

Publications(1)

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse, rat

Applications

WB, IHC, IF/ICC, ELISA

Synonyms

Restin, Melanoma-associated antigen H1, MAGE-H1 antigen, APR-1, APR1

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Tested Applications

• Positive WB detected in: Y79 cells, mouse brain tissue, PC-3 cells
• Positive IHC detected in: human prostate cancer tissue; Note: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0
• Positive IF/ICC detected in: PC-3 cells

Recommended dilution

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochemistry (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Published Applications

• WB: See 1 publications below

Product Information

12424-1-AP targets MAGEH1 in WB, IHC, IF/ICC, ELISA applications and shows reactivity with human, mouse, rat samples.

• Tested Reactivity: human, mouse, rat
• Cited Reactivity: rat
• Host / Isotype: Rabbit / IgG
• Class: Polyclonal
• Type: Antibody
• Immunogen:

  CatNo: Ag3090

  Product name: Recombinant human MAGEH1 protein

  Source: e coli.-derived, PGEX-4T

  Tag: GST

  Domain: 1-219 aa of BC011954

  Sequence: MPRGRKSRRRRNARAAEENRNNRKIQASEASETPMAASVVASTPEDDLSGPEEDPSTPEEASTTPEEASSTAQAQKPSVPRSNFQGTKKSLLMSILALIFIMGNSAKEALVWKVLGKLGMQPGRQHSIFGDPKKIVTEEFVRRGYLIYKPVPRSSPVEYEFFWGPRAHVESSKLKVMHFVARVRNRCSKDWPCNYDWDSDDDAEVEAILNSGARGYSAP

• Full Name: melanoma antigen family H, 1
• Calculated Molecular Weight: 219 aa, 24 kDa
• Observed Molecular Weight: 28 kDa
• GenBank Accession Number: BC011954
• Gene Symbol: MAGEH1
• Gene ID (NCBI): 28986
• RRID: AB_2877856
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Antigen affinity purification
• UNIPROT ID: Q9H213
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Publications

• Human
• Mouse
• Rat
• Monkey
• Pig

Species	Application	Title
rat	WB	Oxid Med Cell Longev; DDAH1/ADMA Regulates Adiponectin Resistance in Cerebral Ischemia via the ROS/FOXO1/APR1 Pathway.; Authors - Yichen Zhao