MAML1 Recombinant monoclonal antibody

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• MAML1 Recombinant monoclonal antibody (87111-1-RR)

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  WB analysis using 87111-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 87111-1-RR (MAML1 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

MAML1 Uni-rAb^® Recombinant Antibody for WB, ELISA

Cat No. 87111-1-RR

Clone No.252237E6

Host / Isotype

Rabbit / IgG

Reactivity

human, mouse

Applications

WB, ELISA

Synonyms

KIAA0200, Mam 1, Mam1, Mam-1, mastermind like 1 (Drosophila)

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Tested Applications

• Positive WB detected in: HeLa cells, HEK-293 cells, MOLT-4 cells, Jurkat ceells, NIH/3T3 cells

Recommended dilution

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

87111-1-RR targets MAML1 in WB, ELISA applications and shows reactivity with human, mouse samples.

• Tested Reactivity: human, mouse
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen:

  Peptide

• Full Name: mastermind-like 1 (Drosophila)
• Calculated Molecular Weight: 108 kDa
• Observed Molecular Weight: 108-120 kDa
• GenBank Accession Number: NM_014757
• Gene Symbol: MAML1
• Gene ID (NCBI): 9794
• RRID: AB_3745347
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: Q92585
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAML1, also named as KIAA0200 and Mam-1 , belongs to the mastermind family. It acts as a transcriptional coactivator for NOTCH proteins. MAML1 has been shown to amplify NOTCH-induced transcription of HES1. It enhances phosphorylation and proteolytic turnover of the NOTCH intracellular domain in the nucleus through interaction with CDK8. MAML1 binds to CREBBP/CBP which promotes nucleosome acetylation at NOTCH enhancers and activates transcription. Induces phosphorylation and localization of CREBBP to nuclear foci. It plays a role in hematopoietic development by regulating NOTCH-mediated lymphoid cell fate decisions. This antibody is specific to MAML1. The calculated molecular weight of MAML1 is 108 kDa, but modified MAML1 is about 120 kDa.