MAN1A1 Recombinant antibody

• 

  WB analysis using 86094-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 86094-1-RR (MAN1A1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HepG2 using 86094-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MAN1A1 antibody (86094-1-RR, Clone: 250444H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

• 

  Affinity and Kinetic Characterization of 86094-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86094-1-RR against Human MAN1A1 were performed. The affinity constant is 1.04 nM.

MAN1A1 Uni-rAb^TM Recombinant Antibody for WB, IF/ICC, ELISA

Cat No. 86094-1-RR

Clone No.250444H2

Host / Isotype

Rabbit / IgG

Reactivity

human

Applications

WB, IF/ICC, ELISA

Synonyms

Alpha 1,2 mannosidase IA, Alpha-1,2-mannosidase IA, EC:3.2.1.113, Man(9)-alpha-mannosidase, Man9-mannosidase

---

Tested Applications

• Positive WB detected in: HepG2 cells, K-562 cells, HEK-293 cells, human urine
• Positive IF/ICC detected in: HepG2 cells

Recommended dilution

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, Check data in validation data gallery.

Product Information

86094-1-RR targets MAN1A1 in WB, IF/ICC, ELISA applications and shows reactivity with human samples.

• Tested Reactivity: human
• Host / Isotype: Rabbit / IgG
• Class: Recombinant
• Type: Antibody
• Immunogen: MAN1A1 fusion protein Ag20060
• Full Name: mannosidase, alpha, class 1A, member 1
• Calculated Molecular Weight: 653 aa, 73 kDa
• Observed Molecular Weight: 68 kDa
• GenBank Accession Number: BC065827
• Gene Symbol: MAN1A1
• Gene ID (NCBI): 4121
• Conjugate: Unconjugated
• Form: Liquid
• Purification Method: Protein A purification
• UNIPROT ID: P33908
• Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol, pH 7.3.
• Storage Conditions: Store at -20°C. Stable for one year after shipment. Aliquoting is unnecessary for -20^oC storage. 20ul sizes contain 0.1% BSA.

Background Information

MAN1A1, also known as HUMM3, HUMM9, MAN9, belongs to the glycosyl hydrolase 47 family. MAN1A1 is a type II transmembrane protein and catalyzes the hydrolysis of three terminal mannose residues from peptide-bound Man(9)-GlcNAc(2) oligosaccharides. MAN1A1 is a tumor-suppressor and low levels of expression of MAN1A1 correlate with poor prognosis in breast cancer patients (PMID: 29381688).